UJI AKTIVITAS ANTIDIABETES EKSTRAK ETANOL BIJI PEPAYA (CARICA PAPAYA L.) DARI EMPAT DAERAH DI JAWA BARAT

• Main Author: A P, Oktaviana
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/66240
• Institution: Institut Teknologi Bandung

Diabetes mellitus is one of the chronic diseases that is the highest cause of death in Indonesia and has experienced an increasing prevalence every year, especially type 2 Diabetes Mellitus. Diabetes mellitus is characterized by increased blood glucose levels due to disturbances in insulin secretion. One of the therapies used for type 2 Diabetes mellitus uses acarbose as an alpha-glucosidase inhibitor. Research on alpha-glucosidase inhibitors in plants is getting a lot of interest because it has side effects and low toxicity, one of which is papaya seeds (Carica papaya L.). This study was conducted to examine the antidiabetic activity of the ethanol extract of papaya seeds (Carica papaya L.) from four areas in West Java. The research began with characterization and phytochemical screening of crude drugs and extracts. Extraction was carried out by maceration method using 96% ethanol solvent until a thick extract and the extract was monitored using thin chromatography. The extract which had the highest inhibitory activity was papaya seed extract (Carica papaya L.) from Tasikmalaya. The IC50 value was 324,37 µg/ml. This extract was continued to the activity screening stage in vivo using the oral glucose tolerance method. The test was carried out by measuring blood glucose levels 30-180 minutes after maltose induction. Blood glucose levels in the extract group at doses of 100, 300, and 600 mg/kgbw did not significantly reduce blood glucose levels (p>0.05). Thus, in general, the ethanol extract of papaya seeds did not have a better antidiabetic effect than acarbose as standard.