ISOLATION OF XANTHINE OXIDASE ENZYME INHIBITORS FROM SIDAGURI (SIDA RHOMBIFOLIA L.)

• Main Author: Tun Islamiyah, Zhaqina
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/66362
• Institution: Institut Teknologi Bandung

Background and purpose: Hyperuricemia is caused by a disturbance in the purine metabolism. The end product of purine metabolism is uric acid. The number of gout arthritis sufferers in Indonesia varies depending on the region. In addition, using synthetic drugs, herbal medicines are also used to decrease uric acid levels, such as Sida rhombifolia L. In China, using of certain parts or the whole Sida rhombifolia L aims to treat gout arthritis, hemorrhoids, kidney disorder and rheumatism. Research using S. rhombifolia leaves showed xanthine oxidase inhibitory activity. This study aimod to isolate compounds xanthine oxidase enzyme inhibitory using the aerial parts of S. rhombifolia. Methods: The sample was collected in Bandung, West Java. Maceration was used 96% ethanol. The isolation steps include fractionation by liquid-liquid extraction method, followed by separation by vacuum liquid chromatography and column chromatography. Each isolation process was monitored using thin layer chromatography. Xanthine Oxidase inhibition testing was carried out using extracts, fractions and isolates using a microplate reader at 290 nm. Identification of compounds was analyzed using UV-visible spectrophotometer, HPLC, NMR and UPLC-ESI-MS/MS. Results: The ethyl acetate fraction was used for the isolation step because the IC50 value of the ethyl acetate fraction was higher than the ethanol extract, n-hexane fraction and water fraction. The structure elucidation of isolate 1 resulted in two bands having maximum wavelengths of 313,87 nm and 266,87 nm. The results of the analysis with mass showed that the m/z value of isolate 1 was a luteolin-7-O-rutinoside with an m/z value was 594. Isolate 2 obtained was rutin because it had the same retention time as the rutin standard. The IC50 values for compounds 1 and 2 were 62,68 ± 0,10 µg/mL and 19,1 ± 0,23 µg/mL, respectively. Conclusion: Two flavonoids have been isolated from the ethanolic extract of S. rhombifolia, namely Luteolin 7-O Rutinoside and Rutin. The IC50 values of both compounds in inhibiting XO activity were 62,68 ± 0,12 µg/mL and 19,1 ± 0,01 µg/mL, respectively.