ANTI-APOPTOTIC STUDY OF PHYTOCHEMICAL COMPOUNDS OF BITTER MELON (MOMORDICA CHARANTIA) AND SPIRULINA (ARTHROSPIRA PLATENSIS) IN ACUTE MYOCARDIAL INFARCTION THROUGH MOLECULAR DOCKING TEST, AND PHARMACOPHORE MODEL MAPPING

• Main Author: Tri Laksono, Bambang
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/66374
• Institution: Institut Teknologi Bandung

Background and objectives: Acute myocardial infarction is a disease resulting from decreased or complete blockade of blood flow to a portion of the heart. Due to the blockage of the coronary arteries, oxygen supply to the myocardium (heart muscle chambers) is decreased, leading to cell death and necrosis of heart cells. Acute myocardial infarction was found to be associated not only with the incidence of cell necrosis, but also with the incidence of cell apoptosis. This study will demonstrate phytochemical compounds in bitter melon and spirulina that play a role in the apoptotic signaling pathway for the treatment of acute myocardial infarction through molecular docking tests, and active site/pharmacophore model mapping. Methods: Co-crystallized ligands, phytochemical compounds of bitter melon and spirulina, and apoptosis receptor proteins were prepared and validated. Phytochemical compounds were molecularly tethered to the receptor protein and analyzed for binding energy, inhibition constant (Ki) value, and binding interaction with amino acid residues of the receptor protein. Results: There are 5 potential phytochemical compounds from bitter melon and spirulina, namely Catechin, Momordicine I, Phytocyanobilin, Naringenin, and Vicine, which have Ki values of less than 10 uM and binding energy values close to those of co-crystallized ligands. In addition, PI3K became the receptor protein with the binding energy value and Ki value of the extract compounds better than the binding energy value and Ki value of the co-crystallized ligand. Conclusion: that Catechin, Momordicine I, Fikosianobilin, Naringenin, and Vicine can affect cell apoptosis signaling pathway especially PI3K receptor protein in the treatment of acute myocardial infarction disease.