#TITLE_ALTERNATIVE#

• Main Author: Annisa Utami (NIM 107 03 022), Ratna
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/6647
• Institution: Institut Teknologi Bandung

Abstract : <br /> <br /> <br /> <br /> DNA encoding for chaperonin 60.1 protein of Mycobacterium tuberculosis had been amplified by polymerase chain reaction (PCR) using M. tuberculosis chromosome as template. The PCR product of 1643 base pairs (bps) in size was ligated into pGEM-T cloning vector and ligation mixture was transformed into Escherichia coli JM 109 to produce recombinant, pGEM-T carrying DNA fragment encoding for chaperonin 60.1. Migration analysis and cleavage using BamHI and EcoRI restriction enzymes were done to recombinant pGEM-T . Results showed that recombinant pGEM-T migrated slower than that of without insert DNA. Single cleavage analysis of recombinant pGEM-T using BamHI or EcoRI produced two digestion patterns, plasmid of 3503 bps in size and plasmid that was not cut by both enzymes. Recombinant plasmid with first pattern was confirmed not to carry DNA fragment encoding for chaperonin 60.1 and plasmid with the second pattern probably contained DNA fragment encoding for chaperonin 60.1 and is further being characterized. <br />