DESIGN AND FABRICATION OF FLOW CELL & CAPILLARY FLUIDIC MODULE USING 3D PRINTING METHOD WITH INTEGRATED SPCE/AUNP CHIP IN FERRICYANIDE (K4[FE(CN)6]) TESTING

• Main Author: Putri, Delpita
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/66638
• Institution: Institut Teknologi Bandung

ABSTRACT DESIGN AND FABRICATION OF FLOW CELL & CAPILLARY FLUIDIC MODULE USING 3D PRINTING METHOD WITH INTEGRATED SPCE/AuNP CHIP IN FERRICYANIDE (K4[Fe(CN)6]) TESTING By DELPITA PUTRI NIM : 18318020 (Undergraduate Program in Biomedical Engineering) Microfluidics is a field of science and technology systems capable of processing or manipulating liquids and gases on a scale of tens to hundreds of micrometers. Microfluidics can be applied in various fields of science ranging from biology, chemistry, information technology and optics. This technology has problems in terms of commercialization and fabrication. This research will focus on the design and fabrication of flow cell and capillary microfluidic platforms using a simpler, more cost-effective method, and allows for faster fabrication results, namely by using the 3D printing method. Previous research has fabricated a microfluidic platform as a flow cell, but when testing the fluid flow there is still leakage. In this final project, design optimization is carried out so that the platform does not leak. The fabricated platform is then integrated with screen printed carbon electrodes or SPCE electrochemical sensors. On the surface of the SPCE, gold nanoparticles were modified by electrodeposition in microtubes and fabricated container platforms. Modifications were made so that the electrodes had optimal electrochemical performance. The morphology of AuNPs formed on the SPCE surface was analyzed by SEM and EDS characterization methods. The SPCE/AuNP was then applied as an electrochemical sensor to detect ferricyanide. Based on the test in the flow cell using the SPCE cyclic voltammetry (CV) technique after being modified in microtubes and containers, the current increase was 47.68% and 48.98% with a difference of peak to peak separation of 0.13 V and 0.14 V. also carried out in capillary microfluidic. When the SPCE modification was carried out in microtubes and containers, it was obtained that there was an increase in current of 39.47% and 38.71% with a difference of peak to peak separation of 0.17 V and 0.15 V. Tests in flow cells and microfluidic capillaries were successfully carried out. Keywords: flow cell, capillary microfluidic, gold nanoparticles, electrodeposition, screen printed carbon electrode, ferricyanide, cyclic voltammtery.