DEVELOPMENT OF BIMETALLIC CUNI METAL-ORGANIC FRAMEWORK MATERIAL FOR ELECTROCHEMICAL IMMUNOSENSOR APPLICATIONS TO DETECT DENGUE VIRUS NS-1 SEROTYPE 3

• Main Author: Astri Lidiawati, Neng
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/67174
• Institution: Institut Teknologi Bandung

Dengue hemorrhagic fever (DHF) is the most common arboviral disease that is spread throughout the world. This disease is caused by the dengue virus and infects humans through the bite of the Aedes aegypti mosquito. This disease is found in tropical and subtropical areas. In Indonesia, the prevalence of this disease is increasing every year. Indonesia is still one of the countries with the highest infection rate among Southeast Asian countries. Therefore, to reduce the rate of dengue virus infection, early detection can be carried out to reduce the risk. This study developed an electrochemical immunosensor based on a modified metal-organic framework (MOF) on the electrode surface to detect dengue virus NS-1 serotype 3. Cu-BTC and CuNi-BTC materials with various ratios of Cu: Ni of 5:1, 3:1, and 1:1 and the addition of 10 wt% triethanolamine (TEOA) as a modulator have been successfully synthesized. The synthesis was performed by co-precipitation at room temperature and dried at 60? for ±20 hours. Material characteristics were reviewed using X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Fourier-Transform Infrared (FTIR), Brunnauer-Emmet Teller (BET), and electrochemical testing. The performance of the material as an electrochemical immunosensor was tested using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The initial test using the CV approach showed that the composition ratio of Cu:Ni = 1:1 resulted in the most optimum reduction and oxidation current values compared to other variations, 19.12 and 13.17 A, respectively. Based on this, MOF with a composition ratio of 1:1 was used for electrochemical testing to detect dengue virus (DENV). The next process is dengue virus detection. The test was carried out using the DPV approach in the linear range of NS-1 protein concentration of 5 ngml-1 - 0.001 ngml-1. From the DPV test, the lowest detection limit or limit of detection (LoD) was 0.77 pgml-1. In addition, selectivity tests were carried out to support the performance of the CuNi-BTC MOF material. The selectivity test was carried out on 4 variations of the DENV serotype, namely serotypes 1, 2, 3, and 4. The test results showed that the CuNi-BTC MOF material had excellent selectivity against NS-1 DENV serotype 3. Further studies were carried out in human serum. This test is carried out for evaluation in clinical applications of immunosensors that have been made. In this test, the variation of the experimental antigen concentration is compared with the test in human serum (real sample). Recovery for variations in the concentration of NS-1 DENV serotype 3 is 0.001; 0.01; 0.1; 1; and 10 ngml-1 were 98%, 94%, 97%, 95% and 98%, respectively. Based on the results of the research that has been carried out, it can be concluded that the electrochemical immunosensor based on the CuNi-BTC-TEOA-3 material that has been made has the potential for further development as a device for the detection of NS-1 DENV serotype 3 antigens.