BIOSYNTHESYS OF ZNO NANOPARTICLE WITH CLOVE EXTRACT AND ANTIIOFILM ACTIVITY TEST

• Main Author: Sukmalovelina, Claritta
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/67266
• Institution: Institut Teknologi Bandung

Microorganism contamination is a problem in various fields, especially health. The presence of microorganisms in biofilms causes antibiotic resistance to increase 1000 times. ZnO nanoparticles have been investigated to have biofilm inhibitory properties. The biological synthesis of nanoparticles is considered potential because of its abundant sources, simple working procedures, and more environmentally friendly. In addition, clove extract contains eugenol which is useful in the synthesis process. Therefore, this study aimed to evaluate the properties, activity, and mechanism of ZnO nanoparticles synthesized with clove extract. The experimental variation used was the ratio of precursor:extract volume of 30:70 and 30:70. The biosynthetic nanoparticles were compared with the nanoparticles produced by the sol-gel method. The results showed that the aeration process produced complex Zn particles, so calcination was carried out to obtain ZnO nanoparticles T. The 30:70 variation of nanoparticles has a hexagonal morphology with sharp corners with a diameter of 26.92 nm, the 70:30 variation has a morphology close to round with a rough side of 34.98 nm in diameter, and the sol-gel method produces a round morphology with a smooth side of 35.69 nm in diameter. . The biosynthetic nanoparticles experienced hard agglomerate while the sol-gel method nanoparticles experienced soft agglomerate. The results of the antibiofilm activity test on Escherichia coli showed that biosynthetic nanoparticles variation 70:30 had the highest activity with 65.6% remaining biofilm at a concentration of 400 mg/L. Spherical nanoparticles have higher antibiofilm activity than hexagonal nanoparticles. Measurement of the concentration of Zn2+ in the supernatant from the antibiofilm test showed a correlation with the percentage of biofilm remaining at high concentrations. The antibiofilm mechanism of the resulting nanoparticles is the release of Zn2+ ions. This is indicated by the results of the measurement of the concentration of Zn2+ in the supernatant of the antibiofilm test results which are in line with the results of the antibiofilm test.