THE EFFECT OF GLYCEROL, ACETATE, AND IRON (III) IONS ON MICROALGAE LIPID INDUCTION OF CHLORELLA PYRENOIDOSA AS BIODIESEL FEEDSTOCK

• Main Author: Halimatusadia, Rizma
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/67420
• Institution: Institut Teknologi Bandung

Lipids from Chlorella pyrenoidosa potential as feedstock for biodiesel. However, the lipid production process starting with the microalgae cultivation stage is often uneconomical and therefore needs to be optimized. Mixotrophic and heterotrophic cultivation modes require organic carbon source up to 50% of the cost of the cultivation medium. Glycerol dan acetate are by-products of biodiesel transesterification dan fermentative hydrogen production, respectively. Both can be used as low-cost organic carbon sources. Then iron ions can also induce lipids because they are involved in lipid synthesis. Experiment using concentration variations of those three nutrients during cultivation is needed to measure the optimal concentration in lipid production. The purpose of this research are to determine the effect of concentration variations of glycerol, acetate, dan iron (III) ions on growth rates dan lipid yields; characterize lipid products; dan determine the kinetics of growth dan lipid production of C. pyrenoidosa. Research included raw material preparation, cultivation, daily nutrient analysis, biomass harvesting, lipid extraction (using the Bligh & Dyer method), dan analysis of the composition dan physicochemical properties of extracted lipids. Cultivation is carried out by varying the concentration of glycerol dan acetate, respectively (3 g/L, 5 g/L, 7 g/L, dan 10 g/L), Besi (III) ion (0 mg/L, 3 mg/L, 5 mg/L dan 7,5 mg/L). During cultivation, daily decreases in nutrient concentrations will be monitored dan periodic biomass analysis dan lipid yield analysis will be carried out. The statistical analysis using t-test analysis showed that the concentration of iron (III) ion significantly decreased the growth rate of biomass but did not significantly affect the lipid and CN recovery. Then, nutrient acetate increased growth rate, decreased lipid and CN gain significantly while nutrient glycerol did not significantly affect these three parameters.