THE ISOLATION OF LIMONOID COMPOUNDS FROM THE SEEDS OF SANTOL FRUIT (SANDORICUM KOETJAPE) AND THEIR ANTIBACTERIAL ACTIVITIES TEST

• Main Author: Zakiy Fadlullah, Muhammad
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/68080
• Institution: Institut Teknologi Bandung

Sandoricum koetjape (Meliaceae family) is an Indonesian native plant that is widely distributed from Sumatra to Papua island. This plant has economic value and benefits as traditional medicines for diarrhea, dysentery, ringworm, fever, inflammation, etc. One of the main secondary metabolites of this family are limonoid compounds. In this family, the limonoid compounds tend to have more complex structures, higher oxidation states, and more varied arrangements than the other families. It is possible that there are still new limonoid compounds that can be isolated from S. koetjape. In this study, the limonoid compounds of S. koetjape seeds was investigated. The seeds were chosen because not many studies have reported their limonoid content and antibacterial activity. Based on the literature, the seed extract of S. koetjape has excellent antibacterial activity against Bacillus subtilis and Pseudomonas aeruginosa so it is possible that the limonoid compounds from S. koetjape seeds have good antibacterial activity as well. The limonoid compounds were isolated, identified their structures by NMR1D and NMR-2D spectroscopy and tested their antibacterial activities by KirbyBauer disc diffusion method. Two new pure compounds were isolated and identified as 30-hydroxy-3-epi-cipadonoid C (1) which is a cipadonoid-type limonoid and 30-dihydrokoetjapin D (2) which is a trijugin-type limonoid. Both compounds were tested their antibacterial activity against Bacilus subtilis ATCC 6633 and Salmonella typhi ATCC 14028. The results showed that in B. subtilis, compound 2 produced a greater inhibition Sandoricum koetjape (Meliaceae family) is an Indonesian native plant that is widely distributed from Sumatra to Papua island. This plant has economic value and benefits as traditional medicines for diarrhea, dysentery, ringworm, fever, inflammation, etc. One of the main secondary metabolites of this family are limonoid compounds. In this family, the limonoid compounds tend to have more complex structures, higher oxidation states, and more varied arrangements than the other families. It is possible that there are still new limonoid compounds that can be isolated from S. koetjape. In this study, the limonoid compounds of S. koetjape seeds was investigated. The seeds were chosen because not many studies have reported their limonoid content and antibacterial activity. Based on the literature, the seed extract of S. koetjape has excellent antibacterial activity against Bacillus subtilis and Pseudomonas aeruginosa so it is possible that the limonoid compounds from S. koetjape seeds have good antibacterial activity as well. The limonoid compounds were isolated, identified their structures by NMR1D and NMR-2D spectroscopy and tested their antibacterial activities by KirbyBauer disc diffusion method. Two new pure compounds were isolated and identified as 30-hydroxy-3-epi-cipadonoid C (1) which is a cipadonoid-type limonoid and 30-dihydrokoetjapin D (2) which is a trijugin-type limonoid. Both compounds were tested their antibacterial activity against Bacilus subtilis ATCC 6633 and Salmonella typhi ATCC 14028. The results showed that in B. subtilis, compound 2 produced a greater inhibition zone value than S. koetjape seed extract because it was possible that compound 2 in S. koetjape seed extract played a role in antibacterial activity. Meanwhile, in S. typhi, S. koetjape seed extract produced a greater inhibition zone value than compound 1 and 2 because it was possible that there were other compounds in S. koetjape seed extract besides compound 1 and 2 which played a role in antibacterial activity. zone value than S. koetjape seed extract because it was possible that compound 2 in S. koetjape seed extract played a role in antibacterial activity. Meanwhile, in S. typhi, S. koetjape seed extract produced a greater inhibition zone value than compound 1 and 2 because it was possible that there were other compounds in S. koetjape seed extract besides compound 1 and 2 which played a role in antibacterial activity.