OPTIMASI KADAR N, P, DAN RASIO N:P UNTUK PRODUKSI BIOMASSA DAN ASTAKSANTIN PADA SPIROGYRA SP. MENGGUNAKAN ANALISIS RESPONSE SURFACE METHODOLOGY (RSM) DAN PEMODELAN KINETIKA
Astaxanthin is a carotenoid compound with very strong antioxidant properties. Astaxanthin is widely used in the beauty and health industry so that the need for astaxanthin continues to increase. This compound can be produced by green algae, including the macroalga Spirogyra sp. which are often fo...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/68468 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Astaxanthin is a carotenoid compound with very strong antioxidant properties.
Astaxanthin is widely used in the beauty and health industry so that the need for
astaxanthin continues to increase. This compound can be produced by green algae,
including the macroalga Spirogyra sp. which are often found in Indonesian
freshwaters. However, the astaxanthin level in Spirogyra sp. is still low. Therefore,
a cultivation method is needed to increase the production of astaxanthin in
Spirogyra sp. This research was conducted to determine the optimum levels of N
and P to increase the production of astaxanthin in Spirogyra sp. Cultivation was
carried out for 14 days on Blue Green medium with an inoculum concentration of
6 g/L. The levels of N/P in the medium were varied to 1.1/0.01; 1.1/0.03; 1.1/0.09;
2.2/0.01; 2.2/0.03; 2.2/0.09; 6.6/0.01; 6.6/0.03; and 6.6/0.09 mM. Sampling of dry
weight of biomass, nitrate content in the medium, and astaxanthin content were
carried out every 3 (three) days. The dry biomass was then extracted by maceration
method using acetone solvent. This extract was then analyzed using High
Performance Liquid Chromatography (HPLC) with a C18 column, HPLC grade
methanol: aqueous (95:5) eluent with a flow rate of 1 mL/min, and a UV-Vis
Photodiode array detector at ? 482 nm. . In this study, the highest accumulation of
biomass was found at levels of N and P of 1.1 and 0.03 mM, namely as much as
485 mg dry weight with a growth rate of 0.2203 day-1. RSM analysis was then
carried out in order to determine the optimum values of N and P levels for the
cultivation of Spirogyra sp. which were 0.83 and 0.04 mM . The highest
accumulation of astaxanthin was found in biomass cultivated in medium with N/P
1.1/0.01 mM, which is 0.067-0.269 mg/g dry weight with the highest accumulation
on the 12th day of cultivation. RSM analysis was also carried out in order to obtain
determine the optimum values of N and P levels for astaxanthin accumulation in
Spirogyra sp. which were 0.94 mM and 0.01 mM. Logistics, Monod, and
Luedeking-Piret modeling for all variations have been carried out to obtain certain
parameters with the values of growth rate. 0.08 to 0.32 day-1, ?max 0.18±0.02 day-
1, kN 68.2±24.2 mg/L, ki 301,8±78,5 mg/L, YN 0.93±0.68 g biomass/nitrate , ?0.36±0.69, ? -0.01±0.02, and kd 0.04±0.03. Based on the results of the study, the
highest astaxanthin productivity was found in the biomass cultivated at levels of N
1.1 mM and P 0.09 mM, which is 0.07?g/cm2/day. In this study, the accumulation
of biomass increased as the N content and N:P ratio decreased. The accumulation
of astaxanthin increased as the levels of N and P decreased. This indicated that
deficiency of N and P could increase the production and productivity of
astaxanthin in Spirogyra sp |
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