ELISITASI KULTUR KALUS GYRINOPS VERSTEEGII (GILG.) DOMKE UNTUK MENINGKATKAN PEROLEHAN DAN PROFIL SENYAWA GAHARU MENGGUNAKAN HOMOGENAT MISELIUM JAMUR TRICHODERMA REESEI
Agarwood is a resin that is produced in the Thymelaeaceae group of plants when an elicitor/signal such as microbes is given to trigger several metabolic pathways to form agarwood-forming compounds. In nature, obtaining agarwood compounds takes a long process up to decades which makes agarwood dif...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/68746 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Agarwood is a resin that is produced in the Thymelaeaceae group of plants when
an elicitor/signal such as microbes is given to trigger several metabolic pathways
to form agarwood-forming compounds. In nature, obtaining agarwood compounds
takes a long process up to decades which makes agarwood difficult to find in the
market and has a high selling value because of low productivity. So it is necessary
to find an alternative to produce agarwood compounds in a shorter time but with
good quality. Several previous studies have shown that the use of tissue culture
from agarwood-producing plants can produce resin after induction, but the
compound profiles of various combinations of plant tissue and elicitors that have
been studied can still be developed further to obtain better agarwood extract
quality. This study used callus induced from Gyrinops versteegii leaves and
cultivated using Murashige & Skoog (MS) liquid media with a combination of 3
ppm NAA and 0.5 ppm BAP hormones. Cultures were elicited for 10 days using a
mycelium homogenate of the fungus Trichoderma reesei. The elicitor variations
were given 0 mg/L, 4 mg/L, 8 mg/L, and 16 mg/L. The callus was extracted using
the maceration method with n-hexane and the largest extract obtained was found
in the elicitor variation of 4 mg/L, which was 7.051 ± 0.32%. Based on the results
of the GC-MS analysis, it was found that various compounds commonly found in
agarwood extract such as hexanedioic acid, bis (2-ethyl hexyl) ester, 1,4-benzene
dicarboxylic acid, bis (2-ethyl hexyl) ester, 2,4-ditert-butyl phenol, phthalic acid
isobutyl octyl ester, dodecane, and 1,2-benzene dicarboxylic acid, bis(2-methyl
propyl) ester. However, there were no sesquiterpene and chromone derivatives.
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