PRELIMINARY DESIGN OF LIPID AND PROTEIN PRODUCTION SYSTEM FROM CHLORELLA VULGARIS THROUGH TWO-STAGE CULTIVATION INTEGRATED WITH ACETONE-BUTANOL-ETHANOL (ABE) PRODUCTION

• Main Author: Jonathan
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/68758
• Institution: Institut Teknologi Bandung

The increasing need for fuel along with the times is one of the drivers of the use of environmentally friendly and renewable fuels. Environmentally friendly renewable energy such as bioethanol can be produced from fermentation using Enterobacter aerogenes. Fermentation was carried out with glycerol as the sole carbon source with concentrations ranging from 10, 15, 20, 25, to 30 g L?1 in 1000 mL glass bottles under controlled conditions at 37°C for 48 hours of fermentation. Liquid samples, during fermentation, were collected to measure substrate concentration, product, biomass, and pH. Enterobacter aerogenes produced optimal growth at a concentration of 30 g/L with a growth rate of 0.08 hours-1. Bulk fermentation at a concentration of 20 g/L resulted in the best yields of carboxylic acid and ethanol with successive gains; 0.06; 0.15; 0.80 g-product/g-glycerol and the highest productivity of lactic acid and acetic acid was achieved by varying the concentration of glycerol 20 g/L with values of 0.01 and 0.03 g/L/hour while the highest productivity was achieved by glycerol concentration of 30 g /L with a value of 0.17 g-ethanol/g-glycerol. Formation of lactic acid, acetic acid, and ethanol products in bulk fermentation correlates with growth associated. Growth modeling was carried out using the Monod equation with max value = 0.063 hour-1, Ks = 5.787, R2 = 0.835 and SSE = 0.132