ANALISIS METABOLIT SEKUNDER DARI KULTUR KALUS DAN SUSPENSI SELTANAMAN KELOR (MORINGA OLEIFERA L.)

• Main Author: Aulia Farhati, Salsabila
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/69147
• Institution: Institut Teknologi Bandung

Moringa (Moringa oleifera L.) is herbal plant used as food and alternative medicine because it has high nutritional content and benefits in almost all parts and has the ability to cure various and some chronic diseases. In addition, the Moringa plant is useful for pregnant, lactating women, and toddlers in fulfilling nutrition. However, the existence of Moringa plants can be threatened because the content metabolites in Moringa plants and excessive use of plant parts used as medicine and sources of nutrition so that efforts need to be made to increase the content of these compounds. One approach is to use tissue culture techniques. This plant contains bioactive compounds such as kaempferol, quercetin, astragalin, hyperoside, rutin, nicotiflorin, and niazirin which are useful for health so it is necessary to analyze these compounds in the resulting culture. This study aims to determine and identify secondary metabolites contained in callus cultures and cell suspensions from Moringa plants. Explants were cultured on MS medium containing various growth regulators and concentrations. The results were dried and extracted. Secondary metabolite profiles were identified using TLC and the UPLC-MRM-MS/MS instrument. Leaf callus culture in MS medium containing 2,4-D 0.5 µg/mL; 0.1 µg/mL identified kaempferol, astragalin, nicotiflorin, hyperoside, rutin, and niazirin compounds. Leaf suspension culture in MS medium containing 2,4-D 0.1 µg/mL, kaempferol, astragalin, nicotiflorin, hyperoside, and rutin were identified. Leaf callus culture in MS medium containing kinetin 0.1 µg/mL + 2,4-D 5 µg/mL; BAP 2 µg/mL + 2,4- D 2 µg/mL identified kaempferol, quercetin, astragalin, nicotiflorin, hyperoside, rutin, and niazirin compounds. Leaf callus culture in MS medium containing BAP 2 µg/mL + NAA 0.5 µg/mL identified astragalin, nicotiflorin, hyperoside, rutin, and niazirin compounds. Stem callus culture and stem cell suspension in MS medium containing 2,4-D 0.1 µg/mL, kaempferol, astragalin, nicotiflorin, hyperoside, rutin, and niazirin compounds were identified.