SKRINING AKTIVITAS INHIBISI ENZIM ?-GLUKOSIDASE LIMA JENIS TUMBUHAN MARGA ZINGIBER DAN ISOLASI SENYAWA AKTIF DARI TUMBUHAN TERPILIH

• Main Author: Kusuma Wardani, Annisa
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/69284
• Institution: Institut Teknologi Bandung

Diabetes mellitus is a disease characterized by increased blood sugar and is one of the most common diseases in the world. One of the ways to treat diabetes mellitus is with ?-glucosidase enzyme inhibitors that work by inhibiting the decomposition and absorption of carbohydrates and inhibiting the breakdown of oligosaccharides into glucose, thereby preventing the absorption of glucose into the blood. This study aims to determine the potency of the ethanol extract of Zingiber rhizome by determining the value of % inhibition and the potency of the ethanol extract and the fraction of selected plant rhizomes, namely Zingiber ottensii as an inhibitor of the ? -glucosidase enzyme by determining the IC50 value. Determination of the inhibition potential of the ? - glucosidase enzyme was carried out using a microplate reader with a wavelength of 405 nm. The best % inhibition value was given by the ethanol extract of Zingiber ottensii rhizome so that it was continued to the next stage. The IC50 value obtained from Zingiber ottensii ethanol extract was 99.64 ± 3.52 µg/mL, from the n-hexane fraction was 22.71 ± 1.65 µg/mL, from the ethyl acetate fraction was 108.13 ± 2.89 µg/mL, and from water ethanol fraction was 696.59 ± 18.95 µg/mL. Of the three fractions, the n-hexane fraction has the smallest IC50 value, so it can be said that the n- hexane fraction has the best activity in inhibiting the ?-glucosidase enzyme. Further fractionation was carried out on the n-hexane fraction using vacuum liquid chromatography method, then continued with fractionation using classical column chromatography method. The resulting fraction from classical column chromatography was isolated by washing method. The isolate from the n- hexane fraction gave ? -glucosidase enzyme inhibitory activity with an IC50 value of 359.02 ± 9.03 µg/mL. Based on the results of the characterization, the isolate was thought to be a steroid/triterpenoid compound.