SKRINING AKTIVITAS INHIBISI ENZIM ?-GLUKOSIDASE TIGA JENIS TUMBUHAN SUKU RUTACEAE DAN ISOLASI SENYAWA AKTIF DARI TUMBUHAN TERPILIH

• Main Author: Qanita
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/69307
• Institution: Institut Teknologi Bandung

Diabetes mellitus is a common metabolic disease characterized by high blood glucose levels. =?íð­ð?ð???» ?-glucosidase enzyme is one of the approaches in the treatment of diabetes. In ??±?ð??????¸??ð?? ?ÿ?????í ??±?±? ??? ???»?????? ®± ± ®???¸ ®? ??-glucosidase inhibitors. This study aimed to determine one of three species from Rutaceae which were Murraya koenigii, Murraya paniculata, and Citrus x aurantiifolia, which had ?í± ­±??ð?íð­ð???? ®?ð?ð???» ?- glucosidase and then isolated the active compounds. The activity test was carried out quantitatively using microplate reader with acarbose as a positive control. The three samples were extracted by maceration with ethanol 96% as a solvent then inhibitory activity of the three extracts were determined at the concentration of 50, 100, and 200 ?ã?mL. The result showed only Murraya koenigii extract had inhibitory activity with inhibition values were 66.27±0.97; 88.43±1.62; and 87.52±1.94% respectively and IC50 values of 34.27±?????ã?mL. Murraya koenigii extract was fractionated by liquid-liquid extraction using n-hexane, ethyl acetate, and water. IC50 values of fractions were 120.65±4.74; 417.95±18.29; and 77.36±3.93 ?ã?mL respectively. Water fraction was fractionated using classical column chromatography method. Subfractions with the best separation were purified using preparative thin-layer chromatography method. Purity test was carried out by two methods; single-development thin-layer chromotography method with three different mobile phase and using TLC densitometry. From purity test, isolate A was obtained with a purity level of 93.64%. Isolate A was characterized by two-dimensional paper chromatography method, TLC densitometry, and Liquid Chromatography-Mass Spectrometry. The result showed that isolate A is a glycoside flavonoid with quercetin as aglycone substituted with two glucose groups. Isolate A was ¸±?±??ð?±¸ »??ð??ð?íð­ð???? ®?ð?ð?? ? ®??®±??? ?ð???» ????g/mL and showed inhibitory value at 23.6±1.23%. Ethanolic extract of Murraya koenigii leaves had the higest Ainhibitory actihvity compared to Murraya paniculata dan Citrus x aurantiifolia. Active compound isolated from the water fraction is glycoside flavonoid compound with quercetin as aglycone substituted with two glucose groups.