UJI AKTIVITAS IMUNOMODULATOR EKSTRAK ETANOL SEREH DAPUR [CYMBOPOGON CITRATUS (DC) STAPF]

• Main Author: Fajriati, Kurnia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/69329
• Institution: Institut Teknologi Bandung

Diseases related to the immune system are increasing day by day. To overcome diseases related to the immune system, immunomodulators are needed which are compounds that can modulate the immune system. One source of immunomodulators is plants such as lemongrass. Lemongrass is traditionally used by the community as a kitchen spice and also to maintain health. This study aimed to examine the immunomodulatory effect of ethanol extract of lemongrass stalks (EELS). The active compounds in lemongrass stems were extracted using 96% ethanol using the reflux method. The immunomodulatory activity of the ethanolic extract of lemongrass stem (EELS) was tested on female BALB/c mice by testing for non-specific and specific immune responses. The effect on non-specific immune responses was determined by carbon clearance test and determination of organ index. The effect on the specific immune response was determined by determination of total antibody titer, delayed-type hypersensitivity test, and determination of IFN-?and IL-2 levels. The results of the carbon clearance test showed that the 3 doses of EELS (375, 750, and 1,500 mg/kg bw) were immunosuppressive, as indicated by the phagocytic index <1. Both doses of EELS (375 and 1,500 mg/kg bw) significantly reduced the spleen index (p<0.05). The 3 doses of EELS did not affect the humoral immune response but could significantly reduce the cellular immune response seen in the delayed-type hypersensitivity test. Of the 3 doses, only EELS 750 mg/kg bw could increase IFN-?titer although not significantly compared to the control. The 3 doses of EELS increased IL-2 levels but only a dose of 750 mg/kg bw was significant to the control (2.478 ± 0.705 vs. 1.402 ± 0.172). The EELS application test on an animal model of rheumatoid arthritis (RA) showed dose 750 and 1.500 mg/kg bw it could significantly inhibit the development of inflammation on day 20. Based on the results of this study, it can be concluded that EELS has immunosuppressive activity.