#TITLE_ALTERNATIVE#

• Main Author: Novi Kurniawan (NIM:107 02 026), Yohanes
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/6935
• Institution: Institut Teknologi Bandung

Abstract: <br /> <br /> <br /> <br /> Chronic hepatitis B and C are lethal infectious diseases and main cause of cirrhosis and liver cancer. Currently, treatment of chronic hepatitis B and C is a combination therapy using interferon alpha2b (IFNo2b) and ribavirin/lamivudine. Therapy using IFNo2b has <br /> <br /> <br /> <br /> been still considered to be very expensive; therefore is not commonly used in majority of chronic Hepatitis B and C patients in Indonesia. This research was intended to synthesize a coding region of synthetic IFNo2b using Thermodynamically Balanced Inside-out (TBIO) <br /> <br /> <br /> <br /> bidirectional synthesis method based on two steps Polymerase Chain Reaction (PCR) and to clone the synthetic construct of IFNo2b to Escherichia coli JM109. Double cleavage analysis using EcoRI and HindIII and PCR analysis of recombinant plasmid showed one band with approximately 587 base pairs (bps) in size. Sequencing analysis from recombinant plasmid showed about 99% nucleotides from total length of insert DNA were <br /> <br /> <br /> <br /> identified as the coding sequence of synthetic IFNo2b. This result proved that coding sequence of synthetic IFNo2b have been cloned in pGEM-T vector and maintained in E. coli JM109.