SYNTHESIS OF SILVER NANOPARTICLES USING DAYAK ONION EXTRACT (ELEUTHERINE BULBOSA (MILL.) URB.) AND ITS ANTIMICROBIAL ACTIVITY TEST

• Main Author: Rahman, Humairani
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/69369
• Institution: Institut Teknologi Bandung

Synthesis of silver nanoparticles (AgNPs) is commonly performed chemically and physically. However, both methods have the disadvantages of low production rates, are expensive, and not being environmentally friendly. This led to the development of a green chemistry method with an environmentally friendly approach based on the bioreduction of Eleutherine bulbosa (Mill.) Urb extracts. Dayak onions contain phenolic compounds in which this secondary metabolite possibly functions as a bio-reductant for the synthesis of AgNPs. AgNPs biosynthesized by green chemistry have a wide application including antimicrobial. This research aims to synthesize AgNPs using Dayak onion extract and optimize AgNPs, characterize the synthesized AgNPs, and determine the antimicrobial activity of AgNPs against Staphylococcus aureus, Pseudomonas aureginosa and Candida albicans. The obtained AgNPs were characterized using spectrophotometry UV-Visble (UV-VIS), particle size analyzer (PSA), Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM) and scanning electron microscopy (SEM). Then, the antimicrobial activity test was performed out using the microdilution method. The most optimal condition of AgNPs synthesis was between a mixture of 0.01% Dayak onion extract at pH 10 and AgNO3 of 0.0003 M in a ratio of 2:1. The results of the characterization of AgNPs were spherical with a size of 20-50 nm. The results of the synthesis of AgNPs showed antimicrobial activity against S.aureus and P.aureginosa bacteria with a minimum inhibitory concentration of 25 ppm, while C. albicans showed a minimum inhibitory concentration of 50 ppm.