EFEK ANTIDIARE FRAKSI DAUN LAMPENI ( ARDISIA ELLIPTICA. THUNB ) DAN PENGUJIAN AKTIVITAS TERHADAP BAKTERI PENYEBAB DIARE

• Main Author: Oktavia Siahaan, Elsa
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/69409
• Institution: Institut Teknologi Bandung

Diarrhea is still a global, regional and national health problem, especially in developing countries such as Indonesia. Diarrhea is the second deadly disease in children under the age of five. Every year around 525,000 children die due to diarrhea. Diarrhea is the condition of defecation with more fluid consistency and frequency three or more times a day within a 24-hour period. Diarrhea is divided into two groups, namely infectious diarrhea (caused by bacteria, viruses or parasites) and non- infectious (caused by bacteria, viruses or parasites). Herbal medicine can be used in the treatment of diarrhea. Duck’s-eyes are used traditionally and their extracts have been shown to have antidiarrheal effects. However, the active fraction/compound that acts as antidiarrheal and antibacterial is unknown. This study aims to determine the antidiarrheal effect of Duck’s-eyes leaves fraction in vivo and determine its antibacterial activity in vitro. In vivo testing was carried out in castor oil-induced diarrhea mice, while antibacterial testing was carried out in vitro using the microdilution method. The in vivo test results showed that the n-hexane fraction could reduce stool consistency at 0 to 1 hour and 1 to 2 hours. Ethyl acetate and water fractions could reduce stool consistency at 0 to 1 hour, 1 to 2 hours, and 2 to 3 hours. The Ethyl acetate and water fractions could reduce frequency of defecation 0-1 hours. The three fractions of Lameni leaves unable to lose stool weight. The minimum inhibitory concentration (MIC) of the n-hexane fraction against all bacteria was 25,000 ppm. The MIC of ethyl acetate fraction against Escherichia coli (E. coli), Salmonella typhi (S. typhi), Shigella dysenteriae (S. dysenteriae), and Pseudomonas aeruginosa (P. aeruginosa) were 12,500 ppm, 12,000 ppm, 25,000 ppm and 50,000 ppm. The MIC fraction of water in Pseudomonas aeruginosa was 50,000 ppm while in the other three bacteria was 12,500 ppm. The minimum killing concentration (MBC) of the n-hexane fraction in Pseudomonas aeruginosa was 25,000 ppm. The MBC for the ethyl acetate fraction against Salmonella typhi and Pseudomonas aeruginosa was 50,000 ppm while the water fraction for all test bacteria was > 50,000 ppm. The fractions of n-hexane, ethyl acetate and water had an antidiarrheal effect in castor oil-induced diarrhea mice and the antibacterial activity against the four tested bacteria was very weak.