EFFECT OF AZOSPIRILLUM BRASILENSE AND KLEBSIELLA PNEUMONIAE ASSOCIATED WITH UPLAND RICE ON N2 FIXING ACTIVITIES

• Main Author: Hala, Yusminah
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/6960
• Institution: Institut Teknologi Bandung

</i><b>Abstract: </b><p align="justify"><i> Effect of <u>Azospirillum brasilense </u>and <u>Klebsiella pneumoniae </u>associated with upland rice on N2 fixing activities This study was performed to observe the associative effect of <u>Azospirillum brasilense</u>, <u>Klebsiella pneumoniae </u>and combination of each with nitrogen dosage on nitrogen fixation by upland rice, and to observe nitrogen fixation activities associated with upland rice by the Acetylene Reduction Assay (ARA). Bacterias <u>K. pneumoniae</u>, and <u>A. brasilense </u>strain SP7 and strain SP27 were used, while fertilizer used were 0, 25, 50 and 75 kg N/Ha. The result revealed that inoculation of nitrogen fixing bacteria to upland rice did not stimulate tillering and plant height, but increased shoot and root dry weight, plant N total production and acetylene reduction activities. Effect of <u>A. brasilense</u> on acetylene reduction abilities was the same as <u>K. pneumoniae</u>, however the effect of <u>A. brasilense</u> on increasing biomass dry weight (shoot and root) and plant N total production revealed tendency to increase compare to <u>K. pneumoniae</u>. Differences in nitrogen fertilizer did not cause significant effect on all parametric measured, except plant N content. Bacteria and nitrogen fertilizer interaction did not cause significant effect on all parametric measured. From regression analysis there were correlation between root dry weight with shoot dry weight, and correlation between root dry weight with plant N total production.</p>