EFFECT OF RED SEAWEED (KAPPAPHYCUS ALVAREZII) EXTRACT SUPPLEMENTATION ON FEED TOWARDS PERFORMANCE AND MICROBIAL COMMUNITY OF WHITELEG SHRIMP (LITOPENAEUS VANNAMEI) DURING NURSERY PHASE

• Main Author: Fitri Nurjannah, Nisrina
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/69942
• Institution: Institut Teknologi Bandung

Red seaweed (Kappaphycus alvarezii) extract is one of the feed supplements which is known to improve shrimp farming performance. However, K. alvarezii extract that is widely used has a powder form so that it is difficult to be consumed properly by shrimp. This study aims to determine the optimum concentration of K. alvarezii feed supplement extract in the suspension form on the bacterial community profile and farming performance of white shrimp (Litopenaeus vannamei). The study was conducted in two stages, namely the concentration optimization of K. alvarezii supplements and the feeding test of K. alvarezii supplemented feeding on L. vannamei PL-10 post-larvae for 14 days. During the feeding test, several analyses such as water quality parameters analysis, shrimp biological parameters analysis, microbial community profile analysis using Total Plate Count, and analysis of the physiological profile of the bacterial community using the Biolog EcoPlateTM followed by Principal Component Analysis were conducted. Concentration optimization results show that lower supplement concentrations have a better effect on shrimp farming performance. Therefore, in the feeding test, three variations were used, which is supplementation with a concentration of 3 gr / kg of feed (Sp1), supplementation with a concentration of 6 gr / kg of feed (Sp2), and control without supplementation (K). Analysis of water quality parameters shows that all treatments are at optimum conditions during the feeding test period. The Sp 1 treatment gave the best growth rate (6.63 ± 1.87 mg / day), feed conversion ratio (1.37 ± 34), productivity (0.517 ± 0.109 kg / m3), and total biomass (5.17 ± 1.10 gr), although it did not differ significant (p> 0.05) from other treatments. The total bacteria in shrimp samples of Sp1 treatment was higher than other treatments (106.8 CFU / mL). Finally, physiological analysis of the microbial community shows that Sp2 has the highest microbial metabolic activity. Principal Component Analysis analysis also shows that the microbial community of Sp1 and Sp2 shrimp has the potential to use several different carbon sources from the K treatment. From this study, it can be concluded that the supplement of K. alvarezii at a concentration of 3 gr / kg overall has the best influence on culture performance and microbial community profiles of L. vannamei.