SCREENING OF KEY GENES RELATED TO CAROTENOID BIOSYNTHESIS IN THE RIPENING PROCESS OF BANANA FRUIT (MUSA ACUMINATA COLLA) CAVENDISH CULTIVARS WITH A TRANSCRIPTOMIC APPROACH
Bananas are one of the important fruits traded to support global food security due to their high nutritional content. Cavendish cultivar banana pulp contains carotene as provitamin A which is essential in lowering the risk of vitamin A deficiency that occurs in many developing countries. In previous...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/70356 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Bananas are one of the important fruits traded to support global food security due to their high nutritional content. Cavendish cultivar banana pulp contains carotene as provitamin A which is essential in lowering the risk of vitamin A deficiency that occurs in many developing countries. In previous research, it is known that carotene compounds play an important role in the process of fruit ripening, but the molecular factors associated with carotene in the ripening process of banana pulp are not widely known. Therefore, this study aims to reveal key genes related to carotenoid biosynthesis during the ripening process of the Cavendish cultivar banana. In this study, transcriptomic studies were conducted using transcriptome data obtained from bananas at two stages, i.e unripe (1st ripening day) and ripe fruit-pulp (7th ripening day) using Ilumina HiSeq® 2000. The transcriptome data analysis method used is Tuxedo and visualized with cummeRbund. The transcripts then further analyzed with KOBAS 3.0 and WEGO 2.0. The results showed that the presence of DEG (Differential Expressed Gene) a total of 3,837 up-regulated and 3,828 down-regulated in both samples includingtranscription factors with p-values ?0.05 and log2 fold change ? |1|. Based on KOBAS 3.0 result, 10 genes related to carotenoid metabolic pathways in Cavendish cultivars have been identified, i.e LCYE, LCYB, ABA8-Ox, and LUT5 are up-regulated while other genes i.e PDS, Z-ISO, CCD, ?-OHase, and ?-OHase are down-regulated in ripe pulp (7th ripening day). In addition to the transcript analysis, the physicochemical condition has been observed and gene expression also confirmed by the qPCR method. The results of the analysis showed that there are 2 genes that consistently up regulated in ripe pulp i.e LCYE and LCYB. This may indicate the involvement of LCYE and LCYB as key genes in the ripening process of bananas.
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