TRANSCRIPTOMIC ANALYSIS OF GYRINOPS VERSTEEGII (GILG.) DOMKE SAPLINGS AFTER FUSARIUM SOLANI INOCULATION

• Main Author: Setiyobudi, Titis
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/70389
• Institution: Institut Teknologi Bandung

Gyrinops versteegii (Gilg.) Domke is Indonesian-native species of agarwood-producing resin. This resin consists of secondary metabolites, especially sesquiterpene and chromone, due to environmental stress, such as physical wounding and microbial infection. Unfortunately, the information regarding the molecular aspect of agarwood biosynthesis in this species is still limited. Therefore, this research is focused on transcriptomic study related to agarwood constituent biosynthesis, especially sesquiterpene. A local strain of Fusarium solani (GSL1) originated from Gorontalo and induced artificial stress on G. versteegii saplings with 48 hours and 28 days incubation. Transcriptomic analysis which consists of total RNA extraction, sequencing, gene annotation, and differential expression analysis was used to compare the expression profile of control and infection treatment groups with two replicates each. Approximately 10 million reads were obtained from the sequencing of each sample and 139 thousands transcripts obtained from assembly. Transcriptomic analysis using UseGalaxy platform showed that the general expression profile of two treatment groups had high similarity to each other compared to the control group. However, the infection of F. solani induced some changes in the gene expression profile in short term period and was maintained until 28 days of infection. About 60% of total transcripts are identic among all groups and 7% are unique for each group. Genes related to sesquiterpene biosynthesis were also found to be significantly upregulated in treatment groups. Those genes consist of WRKY, MYB transcription factor gene, TPS9, and DGUS. WRKY and MYB are important for agarwood-producing species to induce pathogen-related stress response with log 2 fold change value ranging at 4,45 until 9,35. Meanwhile, TPS9 and DGUS will be expressed into germacrene-? synthase and D-guaiene synthase respectively with log 2 fold change value at 7,39 and 6,87 respectively. Those two enzymes are part of the sesquiterpene synthase (SS) family which responsible for sesquiterpene biosynthesis. In conclusion, the infection of F. solani on G. versteegii saplings induced changes in the expression profile and also upregulated expression of some agarwood-related genes such as WRKY, MYB transcription factor gene, TPS9, and DGUS.