PRODUCTION OF LIPASE ITB1.1 IN PICHIA PASTORIS AND ITS UTILIZATION IN BIODIESEL PRODUCTION

• Main Author: HAURIYAH, IFTITAH
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/70417
• Institution: Institut Teknologi Bandung

Biodiesel is one of solutions to replace fossil fuels as the main transportation energy source. Lipase is a potential catalyst in biodiesel synthesis because it has advantages such as no soap formation, high purity product, adaptability to different biodiesel feedstocks, environmentally friendly, and easy to use. One of the alternative sources that can be used in the production of biodiesel is waste cooking oil. In this research, biodiesel production from fresh cooking oil and waste cooking oil has been carried out using lipase enzymes as catalyst. The waste cooking oil in this study was pretreated by filtering, heating, and adding coconut fiber adsorbent. The lipase enzyme used in this study was recombinant lipase ITB1.1. The purification of Lipase ITB1.1 was carried out using Ni-NTA chromatography. The specific activity of the pure enzyme was 5.9581 U/mg with an increased level of purity 22 times compared to crude extract. The transesterification reaction with a lipase enzyme catalyst was carried out at temperature of 60°C for 24 hours with a composition ratio of oil and methanol volume of 1: 4. The biodiesel obtained was characterized using TLC and GC-MS. Its physical properties in the form of density, viscosity and acid number were also determined. The results of the GC-MS analysis showed that biodiesel produced from both fresh cooking oil and waste cooking oil contained methyl ester compounds with various lengths of carbon chain. Based on its physical properties, biodiesel produced from new cooking oil and waste cooking oil met the SNI quality standards. In this research, an electronic practicum module has also been made to assist students in biodiesel production practicum.