Influence of mangostin on the reproductive function of Swiss Webster female mice (Mus musculus)

• Main Author: ADNAN
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/7042
• Institution: Institut Teknologi Bandung

The aim of this experiment was to study the influence of mangostin on the gestation, lactation and estrous cycle of Swiss Webster mice. Mangostin was dissolved in arachis oil and injected subcutaneously. To test the influence on pregnant mice, mangostin was tested on 3 different gestation periods, namely pre-implantation (1-4 gestation day), early implantation (6-9 gestation day), late implantation (14-17 gestation day) periods. Three different doses of mangostin, namely 10, 25, and 50 mg/ kg b.w., were injected to pregnant mice on the 1 st to the 4th gestation day, but to the other 2 groups only 50 mg/kg b.w. of mangostin was used. Mangostin was injected daily during the pregnancy periods tested. Reproductive performance of treated mice was observed on the 18th day of gestation. To lactating female mice, mangostin at a dose of 50 mg/kg b.w. was injected at alternate days during the lactation period, starting on postnatal-0 day. To evaluate the influence of mangostin on the estrous cycle, mangostin was injected at alternate days during 20 days at a dose of 50 mg/kg b:w. starting on the diestrus phase of the mice. Observation was carried out on the percentage of mice having estrous phase and the score of the vaginalsmear of the treated mice. Mangostin was also tested on the uterine growth of 25 days old immature mice which were treated for 3 consecutive days. The results of this study was that mangostin significantly increase the percentages of gestation loss and post-implantation death so that the percentage of implantation and the number of live fetuses decreased. During the lactation period, the body weight of the offspring treated mice was lower than the controls, but nevertheless the number of death fetuses was not different. During the first and second estrous cycle the percentage of mice which were in estrous phase was high, which was also supported by the high score value of the vaginal smear. Mangostin increased the uterine weight and induced the growth of the uterine wail compared to controls. From the results of there expriments it could be concluded that mangostin is estrogenic and could disturbed the gestation of mice when administered during the pre-implantation and early implantation periods and it also disturbs lactation.