FABRICATION OF LASER PRINTED MICROFLUIDIC PAPER-BASED ANALYTICAL DEVICES (LP-µPADS) TO DETECT ARTIFICIAL URINE AND ESCHERICHIA COLI ARTIFICIALLY CONTAMINATED WATER

• Main Author: Febitania, Devina
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/71046
• Institution: Institut Teknologi Bandung

Laser-printed Microfluidic paper-based analytical devices (LP-?PADs) can be utilized to perform micro-scale laboratory analysis making it a point-of-care testing (POCT) technology that is ASSURED (affordable, sensitive, specific, userfriendly, rapid and robust, equipment free and deliverable to end-users). This study aims to make and determine the characteristics of LP?PADs and determine the application of LP-?PADs through colorimetric detection of nitrite tests on artificial urine and detection of Escherichia coli in artificially polluted water. Research methods include the manufacture of LP-?PADs with the laser printing method, characterization of surface topography, liquid confining ability, barrier and channel resolution, barrier stability against surfactants and solvents, and wetting behaviour. Testing for nitrite in artificial urine was carried out using Griess reagent and testing for E. coli was carried out by measuring the ?-galactosidase enzyme using chlorophenyl red ?-galactopyranoside (CPRG). The results showed that the optimum heating time for making LP-?PADs was 60 minutes, with a minimum channel width of 1.5 mm (n=10) and a barrier width of 0.5 mm (n=10). LP-?PADs were able to withstand glycerol, methanol, and DMSO, but were susceptible to tween 80, SDS, and toluene (n=12). The contact angle of water with the LP-?PADs is less than 90° (n=3), which means it is hydrophilic. Nitrite detection in standard solution produced a linear curve at a concentration of 0.01-0.1 mM and a limit of detection (LOD) of 0.0186 mM (n=3). Testing artificial urine with a concentration of 0.02 mM (n=2) gave an error of 1.21% and a concentration of 0.1 mM (n=3) had an error of 5.65%. Detection of E. coli in artificially polluted water with an incubation time of 12 hours resulted in an LOD of 1 CFU/mL (n=3). These results indicate that LP-?PADs can be applied as a diagnostic tool platform for biological samples such as detection of nitrite in urine and detection of E. coli in polluted water.