NEW MUTATION IN HVSII INDONESIAN HUMAN MITOCHONDRIAL DNA
The database of human mitochondrial DNA (mtDNA) sequence plays an important role in many fields, namely forensic, medical, and anthropology. However, the database of Indonesian mtDNA is still limited, especially the database of Hypervariable Segment II (HVSII). The purpose of this research is to...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/71465 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The database of human mitochondrial DNA (mtDNA) sequence plays an important
role in many fields, namely forensic, medical, and anthropology. However, the
database of Indonesian mtDNA is still limited, especially the database of
Hypervariable Segment II (HVSII). The purpose of this research is to gather
information related to the sequence of Indonesian mtDNA, from both primary and
secondary data. The strategy used is sample collection, lyses of sample from mouth
epithelial and blood cell, amplification of the lysate using Polymerase Chain
Reaction (PCR) with HVIIR and HVIIF primers, and mtDNA sequence
determination using Dideoxy Sanger method. All the sequences obtained were
analyzed to determine the amount, type, and position of mutation occurred.
Electrophoresis of six PCR products showed band of 0.4 kb and 1853 bp were
obtained from sequencing. Anaysis of these sequences showed 68 difference based
on CRS, 48 from 68 differences haven’t been published at Mitomap, the differences
are A82G, G85A, G90A, C295A, G69C, G79T, G81T, A82T, T83A, T88G, T89G,
C280A, C296A, G316C, C317G, T321G, C330A, C345G, A350T, A361C, 68.1C,
81.1T, 83.1C, 91.1T, 92.2CG, 118.1G, 119.1T, 291.1A, 309.1C, 309.2CC, 315.1C,
316.1A, T60d, C86d, G229d, A249d, A278d, T282d, C356d. These mutations can be
proposed as specific mutations for Indonesians |
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