(HUMAN MITOCHONDRIAL DNA MUTATION UTILIZING C++ BASED PROGRAMME MITO MUTATION ANALYZER âMMAâ)
Human mitochondrial DNA, due to its maternally inheritance, can be utilized to identify interpersonal relationship and determine the cause of degenerative disease. In order to determine the mutations occurred in the complete genome, each and every nucleotide of 16,569-base pairs human mtDNA have...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/71466 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Human mitochondrial DNA, due to its maternally inheritance, can be utilized to
identify interpersonal relationship and determine the cause of degenerative disease.
In order to determine the mutations occurred in the complete genome, each and every
nucleotide of 16,569-base pairs human mtDNA have to be analyzed. So far, there are
lacks of mutation determination techniques which are capable of resolving the
amount, type, and position of mutations fast and accurately, especially when dealing
with a great amount of samples. In order to solve the problem, a C++ based
programme, named Mito Mutation Analyzer (MMA), has been designed and applied
to analyze 270 samples simultaneously. The range of the amount of mutation per
sample analyzed is 14 to 103 points with the highest occurrence of deletion and
insertion of 15 and 10 points. MMA program and the illustration of its application
presented in this research could give an understanding of its value in the
development of mtDNA research, since the information about mutations could be
obtained in such a short time and presented in compatible form. |
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