ANALYSIS METHODE OF 917 BASE PAIR IN D-LOOP HUMAN MTDNA

• Main Author: Rachmadi, Bambang
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/71497
• Institution: Institut Teknologi Bandung

Genetic mitochondria DNA ( mtDNA ) has a higher mutation. This mutation pattern could have many applications i.e forensic, evolution and genealogy study, particularly the D-loop region, consist of hypervariable segment 1 (HVS1) and hypervariable 2 segment (HVS2). Unfortunately, the research for D-loop region still more work to be done separately that means time to analysis a sample more over. Based on that reason, development the methode for analysis the region is important. In this research, 0,9 kb DNA fragment in Human D-loop mtDNA will be amplification with Polymerase Chain Reaction (PCR). This research beginning with lysis to obtain DNA template then was amplified using Polymerase Chain Reaction (PCR) method, followed by sequencing of the amplified mtDNA using Dideoxy Sanger method to confirm along with nucleotides analysis using SeqmanTM version 4.0.0. Analysis of PCR product showed 0.9 kb single band on the agarose gel electrophoresis. Sample electrophoregraph data show the 0,9 kb nukcleotides sequens is inside to D-loop region and demonstrated 8 mutations after nucleotide sequence compare with Cambridge Reference Sequence (CRS). Because founding that methode, amplification HVI-HVII region work to be done in one step reaction, so analysis D-loop region more effective and efficien.