ELECTROCHEMICAL BIOSENSOR DEVELOPMENT SCREEN PRINTED CARBON ELECTRODE (SPCE) BASED ON GRAPHENE OXIDE AND GOLD NANOPARTICLE (GO/AUNP) NANOCOMPOSITE FOR DOPAMINE DETECTION
Dopamine (DA) is one of the neurotransmitters in the mammalian central nervous system that plays an important role in kidney function, hormonal and cardiovascular systems. The normal value of DA concentration in blood is 0-0.3 nM and in urine is 0.3-3 ?M. The development of sensors for easy, prec...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Project |
| Language: | Indonesia |
| Subjects: | |
| Online Access: | https://digilib.itb.ac.id/gdl/view/71559 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Dopamine (DA) is one of the neurotransmitters in the mammalian central nervous
system that plays an important role in kidney function, hormonal and
cardiovascular systems. The normal value of DA concentration in blood is 0-0.3
nM and in urine is 0.3-3 ?M. The development of sensors for easy, precise and
selective measurement of dopamine can make a major contribution to disease
diagnosis. Nanomaterial-based electrode modification in electrochemical
biosensors has been widely developed and studied due to several significant results,
such as high redox catalytic ability, electrochemical signal enhancement, small
volume size, high and stable chemical properties, superior electron transfer ability,
surface area and also good biocompatibility.
In this study, a Screen Printed Carbon Electrode (SPCE) electrochemical biosensor
based on graphene oxide and gold nanoparticles (GO/AuNP) was developed to
detect dopamine. GO/AuNP was synthesized using the green synthesis method with
clove oil as a reducing agent. Nanomaterials were characterized by Scanning
Electron Microscope, Energy Dispersive X-ray Spectroscopy, X-Ray Diffraction,
and UV-Vis. The drop-casting method was used to modify the carbon working
electrode by taking into account several optimization parameters, such as the ratio
between nanomaterials and nafion and concentration of deposition solutions.
Meanwhile, the electrochemical performance of nanomaterials and dopamine
oxidation were analyzed using cyclic voltammetry and differential pulse
voltammetry. The results showed that the sensor has a good electrocatalytic
response supporting good sensitivity for DA which has a limit of detection (LOD)
of 7.5 nM; limit of quantification (LOQ) of 22.8 nM; and a linear range from 0.1 -
50 ?M with an R2 value of 0.957. |
|---|