ISOLATION, CHARACTERIZATION, AND BIOACTIVITY EVALUATION OF SECONDARY METABOLITE FROM ENDOPHYTIC FUNGUS DIAPORTHE TECTONAE OF MORUS AUSTRALIS LEAF

• Main Author: Saufi Billah An Nimari, Talhah
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/71675
• Institution: Institut Teknologi Bandung

Morus is a genus belongs to Moraceae family, consists of 22 species spread across Asia, Europe, America, Australia and Africa. Morus plants have many benefits, including being used as traditional medicine and also as food for silkworms (Bombyx mori). Morus plants are also known to be rich in phenolic compounds, especially flavonoids, Diels-Alder adducts, stilbenes, and 2-arylbenzofurans with various important bioactivities. In addition to plants, there are other alternative sources which producing bioactive compounds, i.e. plant tissue cultures and also endophytic microorganisms which are considered to be more efficient. Endophytic fungi are fungi that live in colonies in internal plant tissues without causing damage and are known to be in mutual symbiosis with their host organisms, so that endophytic fungi are also able to biosynthesis various secondary metabolites to support host growth. In addition, it is also reported that endophytic fungi can produce different compounds from their hosts which can even be new compounds, such as epoxyquinophomopsins B from Phomopsis sp. with host Morus cathayana which has the same good bioactivity. So far, the investigation of phytochemicals on endophytic fungi from Morus plants has not been widely reported. Therefore, in this study the isolation of endophytic fungi from the Morus australis plant which molecularly identified as Diaporthe tectonae. This endophytic fungus from the genus Diaporthe is the first discovery in the Morus genus. The endophytic fungus D. tectonae was grown on rice media for 4 weeks and then harvested and macerated using ethyl acetate. The ethyl acetate extract was further fractionated and purified using several chromatographic technique including vacuum liquid chromatography (VLC) and gravitacy column chromatography (GCC). From the endophytic fungus D. tectonae have been isolated five pure compounds, NMR and HRMS spectroscopy data led to characterized of one acetate monosaccharide compound, namely 6-Oacetyl-D-glucose, two phenolic derivatives, i.e. 2-(4-hydroxyphenyl)-acetic acid and tyrosol, as well as two steroid compounds i.e. ergosterol and 3?,5?,9?trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one. Cytotoxic activity test against murine leukemia P-388 cells showed that all compounds were active.