PEMBUATAN MEMBRAN BIOREAKTOR EKSTRAK KASAR ENZIM ALPHA AMILASE UNTUK PENGURAIAN PATI

• Main Author: Paramita, Ratih
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/71695
• Institution: Institut Teknologi Bandung

Membrane bioreactor has two main functions, namely as a biocatalyst and a separation medium. The advantages of membrane bioreactor is products selectively passed through the membrane and will result in higher %yield than conventional methods. In this research, membrane bioreactor is prepared by immobilized crude extract ?-amylase onto poly(methyl methacrylate) membrane. Membrane bioreactor crude extract ?-amylase is used to degrade starch producing oligosaccharide. Crude extract ?-amylase is immobilized onto polyacrylamide gel using entrapping method. Results of this research indicate that the immobilized ?-amylase crude extract still able to degrade starch, but the activity is lower than activity of free enzymes. Performance test of membrane bioreactor showing that %rejection to starch molecul is high, but the ability to degrade starch is low. The optimatization is needed to incrase the perfomance of membrane bioreactor.