VARIASI URUTAN NUKLEOTIDA D-LOOP DNA MITOKONDRIA MASYARAKAT SANGIRAN
Nucleotide sequences information plays an important role in historical reconstructions and forensic purposes. Until now, paleoanthropologists has not done any study related to the relationship between mtDNA of the communities of Sangiran and mtDNA of Homo erectus fossil. The purpose of this resea...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/71759 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Nucleotide sequences information plays an important role in historical reconstructions and
forensic purposes. Until now, paleoanthropologists has not done any study related to the
relationship between mtDNA of the communities of Sangiran and mtDNA of Homo erectus
fossil. The purpose of this research is to determine D-loop nucleotide sequences from four
people of Sangiran. The method of this research consist of epithelium cell were used as DNA
template, the DNA was then copied by PCR method, and the nucleotide sequences were
determined by Sanger method. Agarose gel electrophoresis of PCR product showed that
DNA sample size is 0.9 kb. The 451 nucleotides were determined and analyzed. The results
were compared with Cambridge Reference Sequence (CRS) to find mutation positions in
samples. At position 16226 were found the difference between nucleotide sequences of four
samples and CRS. This difference is hypothesized as an identifying mark for nucleotide
sequences of communities of Sangiran.This work is a part of database making for another
research in finding relationship between people of Sangiran and Homo erectus fossil. |
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