THE EFFECT OF FLUORESCENT ISOTHIOCYANATE (FITC) DYES CONCENTRATION IN FABRICATION NATURAL SILICA-BASED NANOPARTICLES METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS DETECTION

• Main Author: Oktafina Sifana, Nining
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/72207
• Institution: Institut Teknologi Bandung

Methicillin resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide, which has emerged over the past 30 years as a leading cause of both nosocomial and community acquired infections. Biosensors can provide rapid, sensitive and selective detection of the presence and number of bacteria in various environments. Herein, a novel fluorescence biosensor was designed for the rapid detection of MRSA based on silica nanoparticle-modified fluoresceince isothiocyanate (FITC). The surface modification of FSiNP was through the hydrosilylation method. Based on the results of Brauner Emmett-Teller (BET) analysis, the surface area of fluorescence silica nanoparticles (FSiNP) obtained was 377.127 m2/g. The results of the X-ray diffraction (XRD) analysis showed that an amorphous phase. Surface modification of fluorescence silica nanoparticles (FISiP) was carried out by the hydroxylilation method to allow bioreceptors vancomycin conjugated with FSiNP (FSiNP– Van). The maximum fluorescence spectrum of FSiNP–Van was recorded at 490 nm and an emission wavelength of 495 – 600 nm. The sensing principle was based on the fluorescence quenching mechanism of FSiNP–Van and this provided a wide linear MRSA concentration range of 30CFU/mL with a limit detection of 10 - 108 CFU/mL. A rapid response time was observed after 20 min of incubation of MRSA with FSiNP–Van. Consequently, with its advantages of sensitivity and speed, the developed biosensor can be a promising platform for MRSA detection n clinical sample.