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Abstract: <br /> <br /> <br /> Earthworm has been used as a fibrinolytic agent in East Asia for several thousand years. The aim of the research was to partially purify and identify fibrinolytic enzyme of the local earthworm Pheretima sp. The enzyme was isolated from both fresh ear...

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Main Author: Hardiany (NIM 205 03 013), Any
Format: Theses
Language:Indonesia
Online Access:https://digilib.itb.ac.id/gdl/view/7256
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Institution: Institut Teknologi Bandung
Language: Indonesia
id id-itb.:7256
spelling id-itb.:72562017-09-27T15:39:41Z#TITLE_ALTERNATIVE# Hardiany (NIM 205 03 013), Any Indonesia Theses INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/7256 Abstract: <br /> <br /> <br /> Earthworm has been used as a fibrinolytic agent in East Asia for several thousand years. The aim of the research was to partially purify and identify fibrinolytic enzyme of the local earthworm Pheretima sp. The enzyme was isolated from both fresh earthworms and earthworm powder, and then followed by fractionation of 40percent saturated ammonium sulfate salt. The ammonium sulfate fraction was further purified using a Sephacryl S300HR column. Proteolytic activity of the enzyme was determined by the azocasein method, and fibrinolytic activity was determined by its ability to degrade fibrin clots. The crude protein extract and the gel fraction were analyzed by sodium dedocyl sulfate-polyacrylamide gel electrophoresis, and a protein band having protease activity was identified by the zymography method. The clear zone on zymogram showed a protease band of both crude extract and gel fraction. The specific ativity of the gel fraction showed 5 fold higher than that of the crude extract. text
institution Institut Teknologi Bandung
building Institut Teknologi Bandung Library
continent Asia
country Indonesia
Indonesia
content_provider Institut Teknologi Bandung
collection Digital ITB
language Indonesia
description Abstract: <br /> <br /> <br /> Earthworm has been used as a fibrinolytic agent in East Asia for several thousand years. The aim of the research was to partially purify and identify fibrinolytic enzyme of the local earthworm Pheretima sp. The enzyme was isolated from both fresh earthworms and earthworm powder, and then followed by fractionation of 40percent saturated ammonium sulfate salt. The ammonium sulfate fraction was further purified using a Sephacryl S300HR column. Proteolytic activity of the enzyme was determined by the azocasein method, and fibrinolytic activity was determined by its ability to degrade fibrin clots. The crude protein extract and the gel fraction were analyzed by sodium dedocyl sulfate-polyacrylamide gel electrophoresis, and a protein band having protease activity was identified by the zymography method. The clear zone on zymogram showed a protease band of both crude extract and gel fraction. The specific ativity of the gel fraction showed 5 fold higher than that of the crude extract.
format Theses
author Hardiany (NIM 205 03 013), Any
spellingShingle Hardiany (NIM 205 03 013), Any
#TITLE_ALTERNATIVE#
author_facet Hardiany (NIM 205 03 013), Any
author_sort Hardiany (NIM 205 03 013), Any
title #TITLE_ALTERNATIVE#
title_short #TITLE_ALTERNATIVE#
title_full #TITLE_ALTERNATIVE#
title_fullStr #TITLE_ALTERNATIVE#
title_full_unstemmed #TITLE_ALTERNATIVE#
title_sort #title_alternative#
url https://digilib.itb.ac.id/gdl/view/7256
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