#TITLE_ALTERNATIVE#
Phalerin, one of active compound of mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.) fruit has been labeled with I-131 for inflammation location tracer. Phalerin was reacted with NaI-131 by utilizing Iodogen kit as I- oxidator. Labeled compound had been evaluated by high performance liquid chroma...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/7296 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Phalerin, one of active compound of mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.) fruit has been labeled with I-131 for inflammation location tracer. Phalerin was reacted with NaI-131 by utilizing Iodogen kit as I- oxidator. Labeled compound had been evaluated by high performance liquid chromatography and thin layer chromatography then purified by size exclussion chromatography. Labeled compound solution as much as 100 μL was injected to normal and inflammed mice to observe its biodistribution. The labeling process had an effectivity of 90.2 ± 2.8% and the purification of the product was achieved up to 96.0 ± 0.4%. Relative accumulation of labeled phalerin was determined by measuring the radioactivity of tissues utilizing Gamma Counter. Labeled phalerin accumulation in inflammed tissue was higher than in normal tissue 1, 4 and 24 hours after intravenous injection as much as 69.2, 26.4, dan 4.7%, respectively. |
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