FARM TRIAL ON APPLICATION OF RED SEAWEED (KAPPAPHYCUS ALVAREZII)-BASED SUPPLEMENTS FOR WHITELEG SHRIMP GROW-OUT CULTURE IN GEROKGAK, NORTH BALI, INDONESIA : WATER MICROBIOME ANALYSIS WITH FLOW CYTOMETRY

• Main Author: Gede Santana Wiratmaja, I
• Format: Final Project
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/73641
• Institution: Institut Teknologi Bandung

Whiteleg shrimp (Litopenaeus vannamei) is an aquaculture commodity that has high production value in Indonesia. Bali in particular has a total 3-12-ton white leg shrimp in 2020. The cultivation of whiteleg shrimp increases every year because of a convenient production system. In Indonesia, the systems still domination by open semi-intensive that has higher risk. The management of feed supplementation is a solution to decrease the negative impact of an open system. Prebiotics from red seaweed (Kappaphycus alvarezii) are chosen to feed supplement of whiteleg shrimp. Red seaweed contains inulin that affects the growth of probiotics. The research was done for whiteleg shrimp cultivation in Gerokgak, North Bali in purpose to evaluating red seaweed on the prebiotics effect to microbiome of rearing water. Microbiome analysed by the Flow Cytometry method, then correlation of chemical and microbial water parameters analysed to biological shrimp perform. The ponds size are 1,000 m3 and 500 m3 with shrimp density is 180 PL/m3 . The prebiotics (product of Sea6Energy Pvt Ltd®, India) were PBD-5 with dose 1g/kg feed, ABD-3 with dose 2g/kg feed, and competitor with dose 2g/kg feed used for supplementation period in 20-60 the day of culture. Statistical analysis used a t-test method with a P<0,05 signification. The results of water chemical quality (pH, TAN, NO2-N, and NO3-N) were in a stable condition, so that it is less toxic for whiteleg shrimp. Microbial water quality indicating prebiotic supplementation, offers the positive correlation in early cultivation period. The prebiotic suppresses pathogen bacteria because has a lower cell size (CZ) parameter. Comparations between prebiotics products show the used of PBD-5 performance better for biological shrimp performance on productivity (2.28±0.12 kg/m3), survival rate (86.5±7.8%), and feed conversion ratio (1,33±0,02). Prebiotics from red seaweed have potency to maintain microbiological water quality to enhance survival rate, feed conversion ratio, and productivity of whiteleg shrimp cultivation.