NANOSTRUCTURED LIPID CARRIER (NLC) FORMULATION OF CENTELLA ASIATICA EXTRACT TO INCREASE ITS XANTHINE OXIDASE INHIBITORY ACTIVITY

• Main Author: Adriana Aridewo, Marcella
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/73705
• Institution: Institut Teknologi Bandung

Xanthine oxidase acts as a catalyzer for purine production which then will form uric acid. An excessive production of uric acid in the body will lead to hyperuricemia and gout disorders. Pegagan herbs extract (Centella asiatica) has a potential to treat gout disorders though its activity is very weak compared to other drugs. The objective of this research was to make an nanostrucutred lipid carrier for pegagan extract (NLC-EP) and increasing its activity as an xanthine oxidase inhibitor. The NLC development started with lipid screening, surfactant, and process optimization. Formula optimization was carried out using Box-Behnken design. The method used to produce NLC-EP was high shear homogenization and ultrasonication. Characterization of the formula include its organoleptic properties, particle size, polydispersity index, zeta potential, pH, and encapsulation efficiency. The optimum formula for NLC-EP contains 0,1% (b/v) pegagan extract, 3% (b/v) lipid with 9:1 stearic acid and oleic acid ratio, and 8% (b/v) surfactant which consists tween 80 and tegocare 165. NLC-EP had an average particle size of 266,10 ± 13,57 nm, spheric particle, polydispersity index 0,303 ± 0,01, pH 5,01 ± 0,02, zeta potential -30,52 mV and 90,81± 3,37% encapsulation efficiency. NLC-EP showed a 50% inhibition of xanthine oxidase in 28,05 ± 0,31 ppm. There was an increase of NLC-EP xanthine oxidase inhibitory compared to the extract. NLC-EP had a physical and chemical stability in 4°C storage condition.