DEVELOPMENT OF STEVIOSIDE PURIFICATION METHOD FROM STEVIA REBAUDIANA BERTONI USING AROMATIC MACROPOROUS RESIN
Stevia (Stevia rebaudiana Bertoni) is a plant producing the sweet compound steviol glycosides with the main compound stevioside, whose leaves have been used as a natural low-calorie sweetener and have various health benefits and industrial applications, thus attracting the attention of consume...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/73813 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Stevia (Stevia rebaudiana Bertoni) is a plant producing the sweet compound steviol glycosides with
the main compound stevioside, whose leaves have been used as a natural low-calorie sweetener
and have various health benefits and industrial applications, thus attracting the attention of
consumers. Research studying stevioside purification in aromatic macroporous resin columns is still
limited. This research was conducted to purify stevioside compounds using aromatic macroporous
resins through dynamic adsorption and desorption methods in a resin column. The results show that
with a flow rate of 3 BV/hour, 573.3 mg of stevioside could be adsorbed on 25 g of wet aromatic
macroporous resin. Stevioside and impurity compounds could be desorbed with gradient elution
using 50-70% and 0-70% of ethanol respectively. The purity of the stevioside compound increased
from 22.65% (w/w) to 62.68% (w/w) after being eluted in a gradient using 0-70% ethanol, and 22.65
± 0.44% to 27.93 ± 0.87% (w/w) after being eluted using 4 BV of 50% ethanol. The purity of stevioside
compounds increased after being adsorbed and desorbed in an aromatic macroporous resin column.
A more optimal elution system and other purification efforts are needed to obtain a higher purity of
stevioside.
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