COMBINATION EFFECTS OF GOTU KOLA (CENTELLA ASIATICA) LEAVES ETHANOL EXTRACT AND ALMOND OIL (PRUNUS DULCIS) AS HAIR GROWTH PROMOTER IN MALE MICE SWISS-WEBSTER STRAIN ANDROGENETIC ALOPECIA MODEL

• Main Author: Feberia, Evania
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/74196
• Institution: Institut Teknologi Bandung

Androgenetic alopecia occurs due to the activity of androgen hormones which is testosterone. Testosterone in hair follicles is converted into 5?-DHT by 5?-reductase type II enzyme which results in a shortened anagen phase and miniaturization of hair follicles. Almonds contain fatty acids that act as type II 5?-reductase inhibitors. Centella asiatica contains the main triterpenoid compounds namely asiaticoside, asiatic acid and other compounds such as flavonoids namely kaemferol, quercetin which is used in overcoming hair loss and baldness. This study aimed to evaluate the effectiveness of almon oil and the combination of almond oil and Centella asiatica leaves in promoting hair growth on mice model of androgenetic alopecia. Hair tonic formulated into nanoemulsions to promote the penetration of hair growth active ingredientsinto the dermal papilla so that the process of cell proliferation and hair growth becomes faster. Furthermore, formulation, optimization, evaluation of preparations (organoleptic, pH) and stability tests (freeze-thaw) were carried out. The best formula of almond oil nanoemulsion was obtained using a combination of Tween 80 and PEG 400 with a ratio of 1:1 and a total surfactant of 30% resulting in a globular size of 198.60 ± 18.78 nm, a polydispersity index of 0.43 ± 0.05, and a pH of 5.20 ± 0.02. While the best formula of almond oil and Centella asiatica leaves ethanol extract nanoemulsion uses a combination of Tween 80 and PEG 400 with a ratio of 1: 1 and a total surfactant of 25% which results in globular size of 96.67 ± 20.52 nm, polydispersity index of 0.49 ± 0.09, and pH 5.03 ± 0.06. The preparation was tested for acute irritation in albino rabbits and did not cause erythema or udema. Based on statistical tests of hair length and hair weight, all hair tonic preparations produced a significant difference (p<0.05) when compared to positive controls. Centella asiatica leaves ethanol extract showed the longest hair growth with an average of 6.22 mm. Almond nanoemulsion showed the highest hair weight with an average of 27.33 mg. This indicates that a single preparation of almond oil and Centella asiatica leaves ethanol extract provides better hair growth activity when compared to the combination. In addition, pegagan ethanol extract preparations and almond nanoemulsions have equivalent effects (P>0.05) with minoxidil as a standard drug.