COMBINATION EFFECTS OF ALMOND OIL (PRUNUS AMYGDALUS) AND ETHANOL EXTRACT OF KATUK LEAVES (SAUROPUS ANDROGYNUS) AS HAIR GROWTH IN MALE MICE SWISS-WEBSTER STRAIN MODEL ANDROGENIK ALOPECIA

• Main Author: Nivio Anglina Maretha, Nadila
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/74222
• Institution: Institut Teknologi Bandung

Androgenic alopecia is a condition of hair growth imbalance resulting in hair loss caused by increased androgen hormones in the body. Katuk leaves and almond oil have been used for generations to treat hair loss. This study aims to evaluate the effect of katuk leaf ethanol extract, almond oil, and a combination of katuk leaf ethanol extract and almond oil to grow hair in the mice model androgenic alopecia. The combination of almond oil and katuk leaf ethanol extract was formulated into nanoemulsion to enhance the penetration into the hair follicles, improve the stability and appearance of the product. Almond oil and katuk leaf ethanol extract nanoemulsion was prepared by using high-energy emulsification method and surfactant combination of tween 80 and PEG 400. Then, the selected nanoemulsion formula was characterized by organoleptic, pH, morphology and stability test using freeze-thaw method. The products were tested for acute dermal irritation in albino rabbits. The effect of nanoemulsion in increasing hair growth was tested in mice by observing the length and weight of hair growing in mice with androgenic alopecia. The results showed that the best almond oil nanoemulsion formulation with a particle size between 100 – 200 nm using a combination of tween 80 and PEG 400 with a ratio of 1:1 and 30% total concentration. The almond oil nanoemulsion has a globular size of 198.6 ± 18.8 nm with a polydispersity index value of 0.426 ± 0.046, pH 5.2. Futhermore, the combination of tween 80 and PEG 400 with a ratio 1:1 and 25% total concentration of surfactants produced nanoemulsions of almond oil and katuk leaf ethanol extract with a globule size of 102.80 ± 26.18 nm, polydispersity index value of 0.40 ± 0.08 and pH 5. Only 5% katuk extract preparation gives a very mild irritating effect on the skin with irritation index value of 0.22 and very mild irritation category. The hair growth test on mice showed that almond oil nanoemulsion had a significantly better hair growth effect (P < 0.05) with 16.48% longer hair length compared to combination of almond oil and katuk leaf ethanol extract nanoemulsion. In addition, the hair growth effect of almond oil nanoemulsion is equivalent to minoxidil used as a standard drug (P > 0.05).