ACTIVITY OF EXTRACTS AND ISOLATES OF TIGARUN PLANTS (CRATAEVA MAGNA DC.) AGAINST FOLLICULITIS CAUSING MICROBES

• Main Author: Hasani, Najwi
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/74230
• Institution: Institut Teknologi Bandung

Skin infections have become a significant health problem in developing countries, including Indonesia, ranking fourth among all diseases that affect nearly one-third of the world's population. One of the skin infections caused by bacteria and fungi is folliculitis, which is the inflammation of the hair follicles. Tigarun (Crataeva magna DC.) is a typical plant from South Kalimantan and can be developed as a natural antimicrobial agent for treating skin infections. Previous studies have shown that tigarun has antimicrobial activity, but research on the chemical study of antimicrobial compounds in tigarun plants is still limited. Therefore, this study aims to determine the antimicrobial activity of tigarun plant extracts and isolates (Crataeva magna DC.) against the microbes causing folliculitis. Extraction was performed using the maceration method with 96% ethanol as the solvent. The antimicrobial activity of the extract was tested using the agar diffusion and microdilution methods. Monitoring of antimicrobial activity against fractions was tested using TLC bioautography. Fractions 51-55 formed white needle-like crystals and showed antimicrobial activity. They were further sub-fractionated using classical column chromatography and purified using recrystallization and preparative TLC techniques to obtain isolates. Compound identification was performed using specific spray reagents. Based on the antimicrobial activity test results using the agar diffusion method, the tigarun flower extract showed better antimicrobial activity compared to the leaf and stem bark extracts, with the highest activity against Staphylococcus epidermidis (12.35±0.35) and the lowest activity against Pseudomonas aeruginosa (9.35±0.39). Testing against the fungus Malassezia furfur only showed activity against the tigarun flower extract at 200 mg/mL (7.40±0.40), while no activity was observed for all tested extracts against Candida albicans. The flower extract's minimum inhibitory concentration (MIC) test results showed 6.25 mg/mL values for both Cutibacterium acnes and Staphylococcus epidermidis. MIC testing was also conducted for the active isolate, isolate 1, which showed 1000 µg/mL values for Cutibacterium acnes and 500 µg/mL for Staphylococcus epidermidis. Based on the characterization test results, the isolate is suspected to be stigmasterol.