UJI EFEK ANTIINFLAMASI PELET IBUPROFEN LEPAS DI KOLON PADAMODEL ARTRITIS SENDI DI TIKUS WISTAR BETINA

• Main Author: Esther, Mesulam
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/74289
• Institution: Institut Teknologi Bandung

Ibuprofen is one of NSAIDs (Non-Steroidal Antiinflammation Drugs) which is commonly known for its adverse effect in causing ulcer and bleeding in upper part of gastrointestinal tract especially when given frequently. The adverse effects of ibuprofen reported due to its activity resulting lack production of gastrointestinal protecting mucus. One strategic approach to delay the release of ibuprofen upon oral administration in upper part of gastrointestinal tract had been developed. The main aim was to reduce the peptic ulcer due to ibuprofen. A previous study on ibuprofen pellet coated with combination of inulin and shellac demonstrated delay release in upper part of gastrointestinal tract. This pellet released ibuprofen in colon due to colon-microbes activity, hence suppressing the ulcer development after daily oral administration of ibuprofen for 14 days in Wistar rats. This study was aimed to test ibuprofen-colonic-release pellet to treat a peripheral inflammation model in rat's joint. Similarly, the ibuprofen pellet was developed as previously then coated using two different coating systems which was aimed to release the ibuprofen based on pH and microbial activity. The anti-inflammatory effect of ibuprofen in coated pellet was tested compared to uncoated pellet and suspension preparation in arthritis model using female Wistar rats. Inflammation in rats was induced by 7 injections of 0.02mL of carrageenan around its right hind joint over 14 days. Ibuprofen was given in different preparations: suspension, uncoated pellet, and coated pellet. Parameters observed in this study were the percentage of inflammatory reduction and total leukocyte counting reduction. Observation and measurements were done everyday for 14 days. The duration of treatment was taken by referring our previous experiment. The result showed that all ibuprofen treated groups showed a significant decrease of inflammation. The ibuprofen coated pellet showed best effect compared to other treated groups especially at day-6 of observation. Total leukocyte counting increased significantly after the induction process but decreased after treatments with all preparations of ibuprofen. However, group given ibuprofen pellet showed most leucocyte reduction. The model to induce joint inflammation in Wistar rat was successfully developed. Ibuprofen showed suppressive effect in this inflammatory model and surprisingly, ibuprofen formulated for colonic release resulted better activity as compared to both uncoated ibuprofen pellet and ibuprofen suspension, especially after six days of medication.