DEVELOPMENT OF TRIANGULAR SILVER NANOPRISM AS ANTIBACTERIAL AGENT FOR ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS

• Main Author: Metta Carini, Jayanti
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/74465
• Institution: Institut Teknologi Bandung

The mechanism of triangular silver nanoprism as an antibacterial agent against two different types of bacteria namely gram-negative bacteria using Escherichia coli and gram-positive using Staphylococcus aureus was tested in this study using the Total Plate Count (TPC) method. So far, antibiotics are still the most commonly used as antibacterials because of their high level of effectiveness. However, over time, the problem of antibiotic resistance which can cause fatal consequences in humans is also growing. One alternative to this problem is the use of metal nanomaterials in various antibacterial applications, usually used in coatings for medical devices, textiles and cosmetics. In this study, silver metal was chosen because of its high antibacterial effectiveness due to the mechanism of silver ions which can damage bacterial membranes. Silver nanoparticles were synthesized into triangular nanoprism forms with the addition of 50 µL, 100 µL, and 150 µL ethylene glycol to determine their effect on the formation of triangular nanoprisms and their activity in killing bacteria. The results showed that the triangular silver nanoprism was successfully synthesized and characterized by TEM and UV-Visible. After antibacterial testing, data was obtained showing that triangular silver nanoprism was able to kill bacteria with the highest effectiveness in the 150 µL ethylene glycol variation, which was around 88.5% for Escherichia coli and 87.6% for Staphylococcus aureus. This research also shows that the higher the level of ethylene glycol used in the synthesis of triangular silver nanoprism, the higher the effectiveness in killing bacteria.