PENINGKATAN PEROLEHAN EKSTRAKSI SENYAWA POLIFENOL DARI DEDAK PADI (ORYZA SATIVA) MENGGUNAKAN PELARUT METANOL DENGAN PERLAKUAN AWAL FERMENTASI SUBSTRAT PADAT MENGGUNAKAN ASPERGILLUS NIGER

• Main Author: Alyssa, Sandra
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/74484
• Institution: Institut Teknologi Bandung

<p align="justify">Rice bran produced in the rice milling process contains polyphenolic compounds such as ferulic acid. These polyphenolic compounds are bound in the plant fiber matrix making it difficult to extract. Solid substrate fermentation can be applied to increase the recovery of polyphenolic compounds in the extraction process using solvents. This research was conducted to determine the optimum time of rice bran fermentation using Aspergillus niger to increase extract yield and extract bioactivity. The results showed that the fermentation process of rice bran with Aspergillus niger reduced the content of hemicellulose, cellulose and lignin respectively by 22.36%, 42.47% and 22.96% after 7 days of fermentation. Solid substrate fermentation using Aspergillus niger can increase the yield of polyphenolic compounds by 32.8% after 7 days of fermentation. Extraction of polyphenolic compounds was carried out by maceration method using 80% methanol at 40°C for 180 minutes. The highest total polyphenol content of polyphenol extract was 6.18 mg GAE/g rice bran obtained on the 7th day of fermentation. The highest ferulic acid content was obtained on the 3rd day of fermentation with a ferulic acid content of 0.130 mg FA/g rice bran. The lowest IC50 value of antioxidant activity of the polyphenol extract was 150.25 ppm obtained on the 7th day of fermentation. Correlation testing was carried out to find correlations between parameters. Correlation analysis showed a strong correlation between total polyphenol content and extract yield.