ANALISIS PROFIL SENYAWA SESKUITERPEN PADA KULTUR PUCUK AQUILARIA MALACCENSIS YANG DIELISITASI MENGGUNAKAN ISOLAT JAMUR ENDOFIT

• Main Author: Raihan Yunita Putri, Annisaa
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/74971
• Institution: Institut Teknologi Bandung

Agarwood is a resin produced by Thymelaceae plants which being stressed by natural or by artificial wounding. Natural stress on agarwood production requires a very long time hence it needs the new innovation for faster agarwood production method which one is co-cultivation elicitation in-vitro. Co-cultivation elicitation is a plant in-vitro cultivation process where plant are also induced by fungi and both cultured in the same medium. Fungi interacted with plants in this method with penetrating themselves through open wound in plants which then triggers plants to produce resin as plant defense mechanism. This resin contains several secondary metabolites in form of fitoalexin compounds such as sesquiterpenes and chromone. Sesquiterpene have beneficial biological activity such as antibacterial, antiinflammation, cytotoxic, and antioxidant. This research involves the co-cultivation elicitation of the shoot of A. malaccensis cultured in liquid MS (Murashige-Skoog) medium which contains the variation of soluble starch concentration in the medium, shoot then biologically elicited using fungi isolate to induce the production of key compound of agarwood (sesquiterpene) for 7 days. This research aims to identify the genus of fungi isolate using dichotomous key, determine the exponential growth phase for fungi isolate, observe the morphology change of the plants due to elicitation, analyzing the carbon sources consumption profile by the plants-fungi culture, and lastly analyzing the volatile compounds produced after plants elicited by fungi. Based on this research findings, fungi isolate is identified as genus Aspergillus. Exponential growth phase of fungal isolate is 3 – 8 days with most suitable carbon source for the growth is sucrose. Elicitation caused some morphological changes in elicited plants such as browning and abscission. Soluble starch consumption profile analysis results the non-elicitation plants utilize starch more than sucrose. On the other hand, the elicited plants has slower carbon utilization rate compared to the control. Shoot culture which cultured in the medium contains 2 g/L of soluble starch grows more efficiently compared to the 6 g/L. Elicited plant-fungi culture produce more volatile compounds compared to the non-elicited plant. Sesquiterpenes identified in the plant extract of this research are listed; for plant cultured in 2 g/L concentration of soluble starch, furanodienone and 7a-Isopropenyl-4,5-dimethyloctahydroindene-4-carboxylic acid exists, for plant cultured in 6 g/L concentration of soluble starch are 7a-Isopropenyl-4,5-dimethyloctahydroindene-4-carboxylic acid, furanodienone, and dehydrocostus lactone, lastly for non-elicited plants there are no sesquiterpene identified.