#TITLE_ALTERNATIVE#

• Main Author: J. Soegihardjo, C.
• Format: Dissertations
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/7503
• Institution: Institut Teknologi Bandung

Abstract: <br /> <br /> <br /> In order to search for potential plants having antihepatotoxic properties, the pharmacological activities and the flavonoids profile of Sonchus oleraceus L. (fam. Compositae) had been investigated. <br /> <br /> <br /> The selection of Sonchus oleraceus L. as the subject of this investigation had been based on the screening of several plants in the family of Compositae, especially those having close relationship with Silybum marianum (L.) Gaertn. which had been used to cure hepatitis, and has been produced comercially under several trade names. <br /> <br /> <br /> Two approaches had been used for the sreening of those plants namely botanical approach and chemical approach. The screening had been carried out amongst three species of Sonchus and one species of Cynara: Sonchus oleraceus L., Sonchus arvensis L., Sonchus asper (L.) Hill. and Cynara scolymus L., while Silybum marianum (L.) Gaertn. had been used as a reference. In the botanical approach, morphological properties of the plants were compared, whereas in the chemical approach the chromatographic properties of the plants extracts were determined. In latter case, thin layer chromatography and two dimensional paper chromatography of ethanolic extracts were compared in relation to flavonoid derivatives. By comparison, based on chromatographic and morphological data thus obtained, Sonchus oZeraceus L. showed characteristic closer to that of Silybum marianum (L.) Gaertn. than the other species. <br /> <br /> <br /> The antihepatotoxic effects of Sonchus oleraceus L. were studied by measuring the hexobarbital induced sleeping time coupled by histopathological examination of the liver of experimental animals using the methods reported by Jun and Chang. The measurements of hexobarbital induced sleeping time indicated that the ethyl acetate, n-butanol, and water fractions of the aqueous methanolic extract of Sonchus oleraceus L. possessed liver protective activity counteracting the carbontetrachloride intoxication. The ethyl acetate fraction exhibited a higher antihepatotoxic effects compared to that of the other fractions, possibly due to higher concentration of the flavonoids. These results were supported by microscopical prints of the liver preparations. <br /> <br /> <br /> Investigations of the flavonoid profiles of Sonchus oleraceus L. were carried out using chromatographic and spectroscopic methods. In the separation of the products of acid hydrolysis of the water soluble fraction, luteolin was isolated and characterized by its melting point 328degreeC, molecular weight 286, and spectroscopic data. <br /> <br /> <br /> The present works on flavonoid compounds of Sonchus oleraceus L. also succeeded in isolating and characterizing other flavonoid derivatives from the ethyl acetate soluble fraction of aqueous methanolic extract. Isolation were carried out using two dimensional preparative paper chromatography and characterization of the isolates proved to be luteolin 7-0-glycoside, apigenin 7-0-glycoside, <br /> <br /> <br /> 3,4,7-trihydm lavone and its derivatives. <br />