OPTIMIZATION OF POLYCAPROLACTONE-BASED 3D SCAFFOLD PREPARATION COMBINED WITH MARINE SPONGES (SPHECIOSPONGIA VAGABUNDA) BIOSILICA FOR BIOCOMPATIBILITY OF HUMAN WHARTON'S JELLY-DERIVED MESENCHYMAL STEM CELL (HWJ-MSC)

• Main Author: Mufiidah, Afifah
• Format: Final Project
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/75092
• Institution: Institut Teknologi Bandung

A bone defect is a damage or abnormality of bone tissue that causes a decrease or loss of bone function. Tissue engineering is a new strategy to repair damaged bone tissue through cell-based therapies involving cell sources, scaffold materials, and bioactive factors. Scaffolds are engineered to have interactive surfaces and biocompatible to support cell attachment and growth. This research focuses on optimizing the fabrication of a biocompatible porous 3D scaffold for Human Wharton's Jelly Mesenchymal Stem Cell (HWJ-MSC). The scaffold was prepared by salt leaching method with polycaprolactone (PCL) combined with biosilica extracted from sea sponge (Spheciospongia vagabunda) through calcination at 600°C. The use of PCL and NaCl crystals as a porogen with the right concentration will determine the porosity and interconnection of the scaffold so that optimization is conducted by using 5% and 10% concentrations of PCL dissolved in chloroform and the addition of NaCl with 250-400 ?m on the size as much as 25%, 50%, and 75%. Scaffold variations include PCL without biosilica and PCL with biosilica (PCL+BS) 20%, 30%, and 50%. Each scaffold variation was seeded with HWJMSC cells passage 8. HWJ-MSC cells morphology on the scaffold was observed using SEM after day 3, and the colorimetric method using MTT assay was performed to determine HWJ-MSC cells proliferation on the scaffold on days 1, 3, 5, and 7. The results showed that 3D scaffolds made with 10% PCL with the addition of 75% NaCl produced more optimal porosity and interconnection with more pores that were more evenly distributed on the scaffolds due to high solution viscosity. HWJ-MSC cells grown on PCL scaffolds were round with no cytoplasmic extension but appeared more spread on PCL + BS scaffolds. MTT results show HWJ-MSC cell proliferation on the PCL+BS scaffold increased on days 1, 3, 5, and 7 compared to the PCL scaffold, which decreased due to the surface condition of the PCL scaffold, which is more hydrophobic, so it is less favorable for cells. The conclusion is that 3D scaffolds made from PCL 10% combined with biosilica from sea sponge (Spheciospongia vagabunda) are biocompatible. The addition of biosilica increases the biocompatibility of PCL scaffolds by providing a more interactive surface for HWJ-MSC cell attachment and proliferation