BIODEGRADATION OF DIETHYL PHTHALATE BY MICROBIAL ISOLATE FROM LEACHATE OF FINAL PROCESSING SITE (A CASE STUDY IN TPA SARIMUKTI)

• Main Author: Irene B G Z Wanggai, Jeane
• Format: Theses
• Language: Indonesia
• Subjects: Teknik saniter dan perkotaan; teknik perlindungan lingkungan
• Online Access: https://digilib.itb.ac.id/gdl/view/75415
• Institution: Institut Teknologi Bandung

Sarimukti Landfill is classified as an old landfill based on several parameters, including the landfill's operational age, pH, COD, BOD?/COD ratio, NH?-N, and heavy metals.One of the issues that can arise in old landfills is the accumulation of plastic waste, which leads to smaller degradation products, namely microplastics. The production of plastic involves the addition of plasticizer additives to make the plastic more flexible, and one of these additives is Phthalate Diester (PAES). This substance can easily leach into the environment from plastic and microplastics. Continuous exposure of living organisms to PAES can lead to serious health problems. One of the PAES compounds that has been identified as a hazardous pollutant is Diethyl phthalate (DEP). The existing concentration of this compound in TPA Sarimukti is 2.4 mg/L. One technology that can be used to remove DEP compounds is biological degradation. Removal can be achieved by utilizing microorganisms, including bacteria, which utilize DEP as a substrate. The acclimatization process is conducted, followed by the isolation of three bacterial strains from the SBS growth medium that have been exposed to DEP. The isolated strains are then analyzed using microscopy and biochemical analysis to determine their species. The identified strains capable of degrading Diethyl phthalate are Serratia Sp, Pseudomonas Sp, and Proteus Sp. After 15 days of testing, it was found that the DEP compound was completely eliminated, reaching 0 ppm from the initial concentration of 2 ppm for Isolate 1 and Isolate 2, and from the initial concentration of 4 ppm for Isolate 2. The biodegradation results demonstrated a significant reduction, with an efficiency range of 40% to 100%. In addition to efficiency results, the ?max values for Isolate 1, Isolate 2, and Isolate 3 were determined as 0.15427/day, 0.2022/day, and 0.0815/day, respectively. The Ks values for Isolate 1, Isolate 2, and Isolate 3 were determined as 0.1428 mg/l, 0.3734 mg/l, and 0.2349 mg/l, respectively. Pseudomonas Sp is the most effective bacterial isolate for degrading DEP compounds, as indicated by the ?max, Ks, and DEP concentration removal efficiency parameters