PENCIL GRAPHITE ELECTRODE MODIFIED WITH MOLECULARLY IMPRINTED POLY(GLYCINE) FOR VOLTAMMETRIC DETERMINATION OF RHODAMINE B
Rhodamine B is a carcinogenic synthetic dye that is widely misused as a food colorant. This compound is electrochemically active and could be detected using voltammetry method. Pencil graphite electrode (PGE) is a working electrode that is categorized as lack of development although having man...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/75449 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Rhodamine B is a carcinogenic synthetic dye that is widely misused as a food colorant. This
compound is electrochemically active and could be detected using voltammetry method. Pencil
graphite electrode (PGE) is a working electrode that is categorized as lack of development
although having many advantages. However, bare PGE is still less sensitive. Thus, in this
research determination of rhodamine B was evaluated using PGE modified by molecularly
imprinted polymer (PGE-MIP) with glycine as its monomer to increase PGE’s sensitivity and
can be used for samples. In this research, the optimum PGE-MIP preparation was carried out
by varying composition ratio of monomer glycine and rhodamine B and varying
electropolymerization cycle. After that, optimization of rhodamine B measurement was carried
out by finding the optimum pH measurement and voltammetry technique. Peak current as a
response of rhodamine B was measured by PGE-MIP which is higher than PGE modified by
nonimprinted polymer (PGE-NIP) and PGE-bare. The optimum conditions for PGE-MIP
preparation were obtained using a molar ratio of rhodamine B and glycine 1:3 with 10
electropolymerization cycles. Meanwhile, the optimum conditions for rhodamine B
measurement were obtained at pH 7 using square wave voltammetry technique. Measurements
of both repeatability and reproducibility tests of 1 mM rhodamine B solution have %RSD of
5.54% (n=30) and 4.45% (n=3). PGE-MIP has a dynamic range in the concentration of 1-
100 ????M with a detection limit of 1.7 ????M. Scan rate analysis shows that the electron transfer
process at the surface electrode is controlled by the diffusion process. The result of rhodamine
B analysisin the crackers sample using PGE-MIP (voltammetric method) was not significantly
different compared with UV-VIS spectrophotometric method. |
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